| Title: | Odorant-Binding and Chemosensory Proteins in Anthonomus eugenii (Coleoptera: Curculionidae) and Their Tissue Expression |
| Author(s): | Lechuga-Paredes P; Segura-Leon OL; Cibrian-Tovar J; Torres-Huerta B; Velazquez-Gonzalez JC; Cruz-Jaramillo JL; |
| Address: | "Colegio de Postgraduados, Campus Montecillo, Mexico-Texcoco Highway, Km. 36.5 Montecillo, Texcoco 56230, Mexico. Koppert de Mexico, Circuito el Marques Norte No. 82, Industrial Park el Marques, Queretaro 76246, Mexico. Bioinformatics and Technologies Department, Solaria Biodata, Antonio Ortega 817, Benito Juarez, Mexico City 03100, Mexico" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "The pepper weevil Anthonomus eugenii is one of the most damaging pests to the pepper crop. To offer alternative management strategies to insecticides, several studies have identified the semiochemicals that are involved in the pepper weevil's aggregation and mating behavior; however, there is no information on its perireceptor molecular mechanism, to date. In this study, bioinformatics tools were used to functionally annotate and characterize the A. eugenii head transcriptome and their probable coding proteins. We identified twenty-two transcripts belonging to families related to chemosensory processes, seventeen corresponding to odorant-binding proteins (OBP), and six to chemosensory proteins (CSP). All results matched with closely related Coleoptera: Curculionidae homologous proteins. Likewise, twelve OBP and three CSP transcripts were experimentally characterized by RT-PCR in different female and male tissues. The results by sex and tissue display the different expression patterns of the AeugOBPs and AeugCSPs; some are present in both sexes and all tissues, while others show expressions with higher specificity, which suggests diverse physiological functions in addition to chemo-detection. This study provides information to support the understanding of odor perception in the pepper weevil" |
| Keywords: | "Humans Animals Male Female *Weevils/genetics *Coleoptera/genetics Odorants Transcriptome *Insecticides Insect Proteins/genetics *Receptors, Odorant/metabolism Phylogeny Gene Expression Profiling RNA-seq Rt-pcr gene expression pattern pepper weevil perirec;" |
| Notes: | "MedlineLechuga-Paredes, Pablo Segura-Leon, Obdulia Lourdes Cibrian-Tovar, Juan Torres-Huerta, Brenda Velazquez-Gonzalez, Julio Cesar Cruz-Jaramillo, Jose Luis eng 765248/Consejo Nacional de Ciencia y Tecnologia (CONACYT)/ Switzerland 2023/02/26 Int J Mol Sci. 2023 Feb 8; 24(4):3406. doi: 10.3390/ijms24043406" |
