| Title: | The human c-fos serum response factor and the yeast factors GRM/PRTF have related DNA-binding specificities |
| Author(s): | Hayes TE; Sengupta P; Cochran BH; |
| Address: | "Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139" |
| ISSN/ISBN: | 0890-9369 (Print) 0890-9369 (Linking) |
| Abstract: | One of the elements that mediates growth factor and serum inducibility of the human c-fos gene is a region of dyad symmetry that lies between nucleotides -320 and -299 of the human gene. A mammalian protein specifically binds to this sequence element and has been termed the serum response factor (SRF). Gel-shift analysis and competition experiments demonstrate that there is a factor in the yeast Saccharomyces cerevisiae that binds specifically to the human c-fos SRE. The methylation interference pattern of the yeast factor is identical to that of the mammalian SRF. Regulatory elements of cell-type-specific genes in yeast have homologies to the c-fos SRE and complete for binding of both the mammalian and yeast factors to the SRE. Antisera to the gene product of the MCM1 locus react with the yeast SRE-binding factor. These data suggest that this yeast protein is closely related or identical to the factors [general regulator of mating type (GRM) and pheromone/receptor transcription factor (PRTF)] that are required for the regulation of cell-type-specific genes in yeast |
| Keywords: | "Cloning, Molecular DNA/genetics DNA, Fungal/genetics Enhancer Elements, Genetic Fungal Proteins/*genetics Genes, Fungal Genes, Mating Type, Fungal Humans Methylation Nuclear Proteins/*genetics Oligonucleotide Probes Saccharomyces cerevisiae/*genetics Sequ;" |
| Notes: | "MedlineHayes, T E Sengupta, P Cochran, B H eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1988/12/01 Genes Dev. 1988 Dec; 2(12B):1713-22. doi: 10.1101/gad.2.12b.1713" |
