« Previous AbstractcDNA cloning and sequence determination of the pheromone biosynthesis activating neuropeptide of Mamestra brassicae: a new member of the PBAN family    Next AbstractFunctional and expression pattern analysis of chemosensory proteins expressed in antennae and pheromonal gland of Mamestra brassicae »

Eur J Biochem

Title:		Characterization of the general odorant-binding protein 2 in the molecular coding of odorants in Mamestra brassicae
Author(s):		Jacquin-Joly E; Bohbot J; Francois MC; Cain AH; Nagnan-Le Meillour P;
Address:		"INRA, Unite de Phytopharmacie et des Mediateurs Chimiques, Versailles, France"
Journal Title:		Eur J Biochem
Year:		2000
Volume:		267
Issue:		22
Page Number:		6708 - 6714
DOI:		10.1046/j.1432-1327.2000.01772.x
ISSN/ISBN:		0014-2956 (Print) 0014-2956 (Linking)
Abstract:		"The general odorant-binding protein 2 of Mamestra brassicae males has been purified from antennal extracts and examined in binding assays with pheromone components of this species and a behavioral antagonist, cis-11-hexadecenol. The protein showed high affinity for the latter compound and no affinity for the pheromone components. In addition, expression of the protein, studied by in situ hybridization, was restricted to the long sensilla trichodea, which house the neuron that responds to cis-11-hexadecenol. The expression in a functionally defined population of sensilla, together with binding specificity and previous electrophysiological data, suggest an unsuspected role for the general odorant-binding protein 2 in M. brassicae. It may be involved in the transduction process for the behavioral antagonist to which neurons are specifically tuned and always cocompartmentalized in long trichodeal hairs, with neurons responding to the major pheromonal compound, cis-11-hexadecenyl acetate. These data are consistent with the involvement of odorant-binding proteins in the fine discrimination between pheromone and antagonist, which is related to avoidance of interspecific mating mistakes"
Keywords:		"Animals Gene Expression Regulation In Situ Hybridization Male Mechanoreceptors/cytology/*physiology Moths *Odorants Pheromones/*physiology RNA, Messenger/genetics Receptors, Odorant/analysis/genetics/*physiology;"
Notes:		"MedlineJacquin-Joly, E Bohbot, J Francois, M C Cain, A H Nagnan-Le Meillour, P eng Research Support, Non-U.S. Gov't England 2000/10/29 Eur J Biochem. 2000 Nov; 267(22):6708-14. doi: 10.1046/j.1432-1327.2000.01772.x"