| Title: | Identification of PBAN-like peptides in the brain-subesophageal ganglion complex of lepidoptera using Western-blotting |
| Author(s): | Jacquin-Joly E; Descoins C; |
| Address: | "Departement de Phytopharmacie, INRA, Versailles, France" |
| DOI: | 10.1016/0965-1748(95)00094-1 |
| ISSN/ISBN: | 0965-1748 (Print) 0965-1748 (Linking) |
| Abstract: | "An immunoblotting technique used to visualize pheromone-biosynthesis-activating-neuropeptide (PBAN)-like peptides in insect tissues is described. This technique involves a tricine-SDS-PAGE system and a chemiluminescent revelation of the antigens. Using this technique, PBAN-like immunoreactive peptides were found in the brain-subesophageal ganglion complex of various lepidopteran species, including moths: Heliothis zea, Mamestra brassicae, Spodoptera littoralis, S. latifascia and S. descoinsi (Noctuidae), Eldana saccharina (Pyralidae), and a butterfly: Pieris brassicae (Pieridae). PBAN-like peptides were detected in both sexes of the species studied, and even in a butterfly species that does not use pheromone to mate. This suggests that those peptides are widely distributed among Lepidoptera and confirms that they could be involved in functions other than regulation of sex pheromone production" |
| Keywords: | "Amino Acid Sequence Animals Blotting, Western Brain Butterflies/*metabolism Electrophoresis, Polyacrylamide Gel Female Ganglia, Invertebrate/*metabolism Glycine/analogs & derivatives Immunoblotting Male Molecular Sequence Data Moths/*metabolism Neuropepti;" |
| Notes: | "MedlineJacquin-Joly, E Descoins, C eng England 1996/02/01 Insect Biochem Mol Biol. 1996 Feb; 26(2):209-16. doi: 10.1016/0965-1748(95)00094-1" |
