| Title: | Autophagy Is Required to Sustain Increased Intestinal Cell Proliferation during Phenotypic Plasticity Changes in Honey Bee (Apis mellifera) |
| Author(s): | Guo Y; Hu R; Li N; Li N; Wu J; Yu H; Tan J; Li Z; Xu S; |
| Address: | "Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China. School of Life Sciences, Capital Normal University, Beijing 100048, China" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Tissue phenotypic plasticity facilitates rapid adaptation of organisms to biotic and/or abiotic pressure. The reproductive capacity of honey bee workers (Apis mellifera) is plastic and responsive to pheromones produced by broods and the queen. Egg laying workers (ELWs), which could reactivate their ovaries and lay haploid eggs upon queen lost, have been commonly discussed from many aspects. However, it remains unclear whether midgut homeostasis in ELWs is affected during plastic changes. Here, we found that the expression of nutrition- and autophagy-related genes was up-regulated in the midguts of ELWs, compared with that in nurse workers (NWs) by RNA-sequencing. Furthermore, the area and number of autophagosomes were increased, along with significantly increased cell death in the midguts of ELWs. Moreover, cell cycle progression in the midguts of ELWs was increased compared with that in NWs. Consistent with the up-regulation of nutrition-related genes, the body and midgut sizes, and the number of intestinal proliferation cells of larvae reared with royal jelly (RJ) obviously increased more than those reared without RJ in vitro. Finally, cell proliferation was dramatically suppressed in the midguts of ELWs when autophagy was inhibited. Altogether, our data suggested that autophagy was induced and required to sustain cell proliferation in ELWs' midguts, thereby revealing the critical role of autophagy played in the intestines during phenotypic plasticity changes" |
| Keywords: | "Bees/genetics Animals Larva/physiology *Intestines *Autophagy/genetics Adaptation, Physiological Cell Proliferation autophagy honey bee (Apis mellifera) midgut phenotypic plasticity;" |
| Notes: | "MedlineGuo, Yueqin Hu, Ruoyang Li, Naikang Li, Nannan Wu, Jiangli Yu, Huimin Tan, Jing Li, Zhouhua Xu, Shufa eng CARS-44-KXJ6/Modern Agro-Industry Technology Research System/ CAAS-ASTIP-2021-IAR/Agricultural Science and Technology Innovation Program/ Switzerland 2023/02/12 Int J Mol Sci. 2023 Jan 18; 24(3):1926. doi: 10.3390/ijms24031926" |
