Methods Enzymol
2022 Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids Detection and analysis of novel and known plant volatile apocarotenoids Identification of volatile producing enzymes in higher fungi: Combining analytical and bioinformatic methods 2020 Site-directed mutagenesis of odorant-binding proteins Gravimetric biosensors Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins Effect of OBPs on the response of olfactory receptors 2019 "Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor" 2015 A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs 2010 "Assessment of constitutive activity of a G protein-coupled receptor, CPR2, in Cryptococcus neoformans by heterologous and homologous methods" Strategies for isolating constitutively active and dominant-negative pheromone receptor mutants in yeast 2009 "Expression of CXCR4, a G-protein-coupled receptor for CXCL12 in yeast identification of new-generation inverse agonists" 2004 Analysis of RGSZ1 protein interaction with Galphai subunits Yeast-based screening for inhibitors of RGS proteins Identification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection Mathematical modeling of RGS and G-protein regulation in yeast 2001 "Purification of functional Sec13p-Sec31p complex, a subunit of COPII coat" 2000 "Yeast surface display for directed evolution of protein expression, affinity, and stability" 1998 Functional assays for analysis of yeast ste6 mutants 1997 Functional analysis of FAR1 in yeast Alpha-factor synchronization of budding yeast 1995 Lipid-mediated a-factor interactions with artificial membranes 1992 Reconstitution of endoplasmic reticulum to Golgi transport in yeast: in vitro assay to characterize secretory mutants and functional transport vesicles Purification of yeast Sec23 protein by complementation of mutant cell lysates deficient in endoplasmic reticulum-to-Golgi transport Use of sec mutants to define intermediates in protein transport from endoplasmic reticulum Reconstitution of transport from endoplasmic reticulum to Golgi complex using endoplasmic reticulum-enriched membrane fraction from yeast 1991 Assay of yeast mating reaction Yeast endocytosis assays Genetic screens and selections for cell and nuclear fusion mutants
