2022

Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids

Detection and analysis of novel and known plant volatile apocarotenoids

Identification of volatile producing enzymes in higher fungi: Combining analytical and bioinformatic methods

2020

Site-directed mutagenesis of odorant-binding proteins

Gravimetric biosensors

Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing

Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography

Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects

Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins

Effect of OBPs on the response of olfactory receptors

2019

"Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor"

2015

A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs

2010

"Assessment of constitutive activity of a G protein-coupled receptor, CPR2, in Cryptococcus neoformans by heterologous and homologous methods"

Strategies for isolating constitutively active and dominant-negative pheromone receptor mutants in yeast

2009

"Expression of CXCR4, a G-protein-coupled receptor for CXCL12 in yeast identification of new-generation inverse agonists"

2004

Analysis of RGSZ1 protein interaction with Galphai subunits

Yeast-based screening for inhibitors of RGS proteins

Identification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection

Mathematical modeling of RGS and G-protein regulation in yeast

2001

"Purification of functional Sec13p-Sec31p complex, a subunit of COPII coat"

2000

"Yeast surface display for directed evolution of protein expression, affinity, and stability"

1998

Functional assays for analysis of yeast ste6 mutants

1997

Functional analysis of FAR1 in yeast

Alpha-factor synchronization of budding yeast

1995

Lipid-mediated a-factor interactions with artificial membranes

1992

Reconstitution of endoplasmic reticulum to Golgi transport in yeast: in vitro assay to characterize secretory mutants and functional transport vesicles

Purification of yeast Sec23 protein by complementation of mutant cell lysates deficient in endoplasmic reticulum-to-Golgi transport

Use of sec mutants to define intermediates in protein transport from endoplasmic reticulum

Reconstitution of transport from endoplasmic reticulum to Golgi complex using endoplasmic reticulum-enriched membrane fraction from yeast

1991

Assay of yeast mating reaction

Yeast endocytosis assays

Genetic screens and selections for cell and nuclear fusion mutants