2022

Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery

2019

Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5

Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine

2018

Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction

Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating

2017

Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion

Spatial and temporal signal processing and decision making by MAPK pathways

TORC1 signaling exerts spatial control over microtubule dynamics by promoting nuclear export of Stu2

2015

Pheromone-encoding mRNA is transported to the yeast mating projection by specific RNP granules

2010

Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae

2009

Antagonistic regulation of Fus2p nuclear localization by pheromone signaling and the cell cycle

2008

Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast

Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating

2007

The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating

YGR198w (YPP1) targets A30P alpha-synuclein to the vacuole for degradation

2006

Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit

Bqt2p is essential for initiating telomere clustering upon pheromone sensing in fission yeast

Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation

2005

Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase

Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast

2004

A conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1

Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators

"Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway"

Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p

2003

Cdc48p is required for the cell cycle commitment point at Start via degradation of the G1-CDK inhibitor Far1p

Dynamic phosphoregulation of the cortical actin cytoskeleton and endocytic machinery revealed by real-time chemical genetic analysis

2002

The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast

"Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis"

The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint

2001

Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation

UGO1 encodes an outer membrane protein required for mitochondrial fusion

2000

Dynamic localization of protein phosphatase type 1 in the mitotic cell cycle of Saccharomyces cerevisiae

"Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASp"

Nucleocytoplasmic shuttling of the Cdc42p exchange factor Cdc24p

The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae

Recycling of the yeast a-factor receptor

"Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating"

1999

The GTP-binding protein Rho1p is required for cell cycle progression and polarization of the yeast cell

"Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p"

A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating

"Chs7p, a new protein involved in the control of protein export from the endoplasmic reticulum that is specifically engaged in the regulation of chitin synthesis in Saccharomyces cerevisiae"

"Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function"

1998

Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization

"Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton"

"A large PEST-like sequence directs the ubiquitination, endocytosis, and vacuolar degradation of the yeast a-factor receptor"

Dual roles for Ste24p in yeast a-factor maturation: NH2-terminal proteolysis and COOH-terminal CAAX processing

Rvs161p interacts with Fus2p to promote cell fusion in Saccharomyces cerevisiae

A role for a protease in morphogenic responses during yeast cell fusion

Pheromone-regulated genes required for yeast mating differentiation

Role of the yeast Gin4p protein kinase in septin assembly and the relationship between septin assembly and septin function

Reconstitution of retrograde transport from the Golgi to the ER in vitro

1997

KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion

Biogenesis of the Saccharomyces cerevisiae mating pheromone a-factor

"A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor"

Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant

"SOI1 encodes a novel, conserved protein that promotes TGN-endosomal cycling of Kex2p and other membrane proteins by modulating the function of two TGN localization signals"

Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p

1996

Cell fusion during yeast mating requires high levels of a-factor mating pheromone

Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro

"Assembly of ER-associated protein degradation in vitro: dependence on cytosol, calnexin, and ATP"

Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing

Role of the ABC transporter Ste6 in cell fusion during yeast conjugation

Ubiquitination of the yeast a-factor receptor

The sequence NPFXD defines a new class of endocytosis signal in Saccharomyces cerevisiae

Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding

"Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)"

1995

Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation

"DNM1, a dynamin-related gene, participates in endosomal trafficking in yeast"

"Glycosyl phosphatidylinositol-dependent cross-linking of alpha-agglutinin and beta 1,6-glucan in the Saccharomyces cerevisiae cell wall"

FAR1 is required for oriented polarization of yeast cells in response to mating pheromones

The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2

Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients

VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae

"Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae"

1994

Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes

Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein

"Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast"

1993

Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors

The a-factor transporter (STE6 gene product) and cell polarity in the yeast Saccharomyces cerevisiae

end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae

Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast

Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70

A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome

Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment

1992

"Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast"

Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport

Selective and immediate effects of clathrin heavy chain mutations on Golgi membrane protein retention in Saccharomyces cerevisiae

1991

Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site

Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae

The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle

MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication

The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles

Sequence of the clathrin heavy chain from Saccharomyces cerevisiae and requirement of the COOH terminus for clathrin function

A role for unsaturated fatty acids in mitochondrial movement and inheritance

1990

Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast

Identification and initial characterization of an autocrine pheromone receptor in the protozoan ciliate Euplotes raikovi

Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast

The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating

1989

"A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus"

Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast

Secretion in yeast: preprotein binding to a membrane receptor and ATP-dependent translocation are sequential and separable events in vitro

1988

Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated

Protein transport to the vacuole and receptor-mediated endocytosis by clathrin heavy chain-deficient yeast

Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant

1987

Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection

1986

Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor

Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis

1985

Defective temporal and spatial control of flagellar assembly in a mutant of Chlamydomonas reinhardtii with variable flagellar number

1984

Bud formation by the yeast Saccharomyces cerevisiae is directly dependent on 'start'

Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes

1980

Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae

Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone

Cell cycle phase expansion in nitrogen-limited cultures of Saccharomyces cerevisiae

1976

Functional significance of the variations in the geometrical organization of tight junction networks

1968

Morphological and functional aspects of an insect epidermal gland

1962

Polyester-methacrylate embedments for electron microscopy