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Food Res Int

Title:		"Effect of alkaline extraction pH on structure properties, solubility, and beany flavor of yellow pea protein isolate"
Author(s):		Gao Z; Shen P; Lan Y; Cui L; Ohm JB; Chen B; Rao J;
Address:		"Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA. Edward T. Schafer Agricultural Research Center, Cereal Crops Research Unit, Hard Spring and Durum Wheat Quality Lab, USDA-ARS, Fargo, ND 58108, USA. Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA. Electronic address: Jiajia.rao@ndsu.edu"
Journal Title:		Food Res Int
Year:		2020
Volume:		20200129
Issue:
Page Number:		109045 -
DOI:		10.1016/j.foodres.2020.109045
ISSN/ISBN:		1873-7145 (Electronic) 0963-9969 (Linking)
Abstract:		"In the current study, the impact of alkaline extraction pH (8.5, 9.0, and 9.5) on chemical composition, molecular structure, solubility and aromatic profile of PPI was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the quantification of free sulfhydryl group and disulfide bond contents, size exclusion chromatography with multi-angle static light scattering and refractive index (SEC-MALS-RI), circular dichroism (CD) spectroscopy, and headspace solid phase micro extraction gas chromatography-mass spectroscopy (HS-SPME-GC-MS). We found that protein recovery yield increased from 49.20% to 57.56% as the alkaline extraction pH increased from 8.5 to 9.5. However, increasing the extraction pH promoted the formation of protein aggregates which decreased the percent protein solubility although there was no influence on protein secondary structure. PPI extracted at pH 9.0 possessed the lowest beany flavor as revealed by the selected six beany flavor markers including alcohols, aldehydes, ketones and pyrazine. The lowest lipoxygenase activity at pH 9.0 may contribute to the least beany flavor in PPI. Therefore, pH 9.0 was found to be the optimal condition for preparing premium PPI in terms of yield, functionality, and aromatic profile using alkaline extraction-isoelectric precipitation process. The findings could have fundamental implications for the preparation and utilization of pea proteins in food applications"
Keywords:		"Circular Dichroism Disulfides/analysis Food Handling Gas Chromatography-Mass Spectrometry *Hydrogen-Ion Concentration Lipoxygenase/metabolism Odorants/analysis Pea Proteins/*chemistry/*isolation & purification Peas/*chemistry Protein Structure, Secondary;"
Notes:		"MedlineGao, Zili Shen, Peiyi Lan, Yang Cui, Leqi Ohm, Jae-Bom Chen, Bingcan Rao, Jiajia eng Research Support, U.S. Gov't, Non-P.H.S. Canada 2020/04/06 Food Res Int. 2020 May; 131:109045. doi: 10.1016/j.foodres.2020.109045. Epub 2020 Jan 29"