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BMC Plant Biol

Title:		Metabolome and transcriptome analyses identify the plant immunity systems that facilitate sesquiterpene and lignan biosynthesis in Syringa pinnatifolia Hemsl
Author(s):		Gao J; Li T; Jiao L; Jiang C; Chen S; Huang L; Liu J;
Address:		"Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 301617, Tianjin, China. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China. Research Institute of Wood Industry, Chinese Academy of Forestry, 100091, Beijing, China. Alashan Mongolian Hospital, Alashan East Banner of Alashan, 75030, Inner Mongolia, China. Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 301617, Tianjin, China. huangluqi01@126.com. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China. huangluqi01@126.com. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China. juanliu126@126.com"
Journal Title:		BMC Plant Biol
Year:		2022
Volume:		20220322
Issue:		1
Page Number:		132 -
DOI:		10.1186/s12870-022-03537-5
ISSN/ISBN:		1471-2229 (Electronic) 1471-2229 (Linking)
Abstract:		"BACKGROUND: Syringa pinnatifolia Hemsl. is a shrub belonging to the Oleaceae family. The peeled woody stems and roots of S. pinnatifolia are used in Chinese traditional medicine. This plant has been used for centuries, and modern pharmacological research has revealed its medicinal value. However, the wild populations of S. pinnatifolia have been decreasing, and it has been listed as an endangered plant in China. To elucidate the molecular mechanism leading to the synthesis of the major components of S. pinnatifolia for its further development and sustainable use, this study compared peeled stems and twigs at the metabolic and molecular levels. RESULTS: Peeled stems with the purple substance visible (SSP) and peeled twigs without the purple substance (TSP) were compared at different levels. Microscopic observation showed resin-like fillers in SSP and wood fiber cell walls approximately 1.0 mum thicker than those in TSP (wood fiber cell thickness approximately 2.7 mum). In addition, 104 volatile organic compounds and 870 non-volatile metabolites were detected in the non-targeted and widely-targeted metabolome analyses, respectively. Among the 76 differentially accumulated metabolites (DAMs) detected, 62 were up-accumulated in SSP. Most of these DAMs were terpenes, of which 90% were identified as sesquiterpenes in the volatile organic compound analysis. In the analysis of the non-volatile metabolites, 21 differentially accumulated lignans were identified, of which 18, including five subtypes, were accumulated in SSP. RNA sequencing revealed 4,421 upregulated differentially expressed genes (DEGs) and 5,522 downregulated DEGs in SSP compared with TSP, as well as 33,452 genes that were not differentially expressed. Analysis of the DEGs suggested that sesquiterpenes and lignans were mostly biosynthesized via the mevalonate and phenylpropanoid pathways, respectively. Additionally, in SSP, the enriched Gene Ontology terms included response to biotic stimulus and defense response, while the enriched Kyoto Encyclopedia of Genes and Genomes pathways included plant-pathogen interaction and many other pathways related to plant immunity. CONCLUSIONS: This study provides metabolome and transcriptome information for S. pinnatifolia, suggesting that biotic stimuli, including pathogens, are potential and valuable approaches to promoting the biosynthesis of the metabolites linked to the medicinal properties of this plant"
Keywords:		Gene Expression Profiling *Lignans Metabolome/genetics Plant Immunity *Sesquiterpenes *Syringa/genetics Lignan Metabolome Sesquiterpene Syringa pinnatifolia Hemsl.Transcriptome;
Notes:		"MedlineGao, Jiaqi Li, Tianxiao Jiao, Lichao Jiang, Chao Chen, Suyile Huang, Luqi Liu, Juan eng CI2021A04101/CACMS Innovation Fund/ ZZ13-YQ-093-C1/Fundamental Research Funds for the Central public welfare research institutes/ England 2022/03/24 BMC Plant Biol. 2022 Mar 22; 22(1):132. doi: 10.1186/s12870-022-03537-5"