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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		"An LC-MS/MS method to profile urinary mercapturic acids, metabolites of electrophilic intermediates of occupational and environmental toxicants"
Author(s):		Frigerio G; Mercadante R; Polledri E; Missineo P; Campo L; Fustinoni S;
Address:		"EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address: gianfranco.frigerio@unimi.it. EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address: rosa.mercadante@unimi.it. EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address: elisa.polledri@policlinico.mi.it. EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address: pasquale.missineo@policlinico.mi.it. EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address: laura.campo@policlinico.mi.it. EPIGET - Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health, University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, via S. Barnaba, 8, 20122 Milan, Italy. Electronic address: silvia.fustinoni@unimi.it"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2019
Volume:		20190406
Issue:
Page Number:		66 - 76
DOI:		10.1016/j.jchromb.2019.04.015
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		"INTRODUCTION: Mercapturic acids are urinary metabolites of occupational and environmental toxicants. The aim of this work was to develop and validate an analytical assay for the determination of several urinary mercapturic acids to be used as biomarkers of exposure. METHOD: An isotope dilution tandem mass spectrometric method, coupled with reversed-phase liquid chromatography, was developed for the analysis of mercapturic acids derived from several compounds, including those of benzene, toluene, 1,3-butadiene, styrene, acrylonitrile, 4-chloronitrobenzene, acrylamide, acrolein, propylene oxide, N,N-dimethylformamide, crotonaldehyde, ethylene oxide, and methylating and ethylating agents. Samples were prepared by simple filtration after dilution. A validation was carried out, including the assessment of calibration curves, sensitivity, accuracy, precision, process efficiency, and stability, along with external verification. The assay was applied to the analysis of 14 end-of-shift urine samples from unexposed workers and gasoline station attendants. RESULTS: The chromatographic run lasted 18?ª+min. Limits of quantitation ranged from 0.01 to 3.2?ª+mug/L; precision, expressed as relative standard deviation, ranged from 0.6 to 20.9%; and accuracy ranged from 93.4 to 114.9% of theoretical values. The use of deuterated internal standards was suitable for control of the matrix effect. The assay allowed the simultaneous quantitation of urinary mercapturic acids at different concentration ranges. The external verification exercise produced good results. The application of the assay to urine samples from workers revealed differences in mercapturic acid profiles in agreement with the expected patterns of exposure. CONCLUSION: This high-throughput method is valid and useful for the quantitation of urinary mercapturic acids, and is suitable for human biomonitoring of occupational and environmental exposure"
Keywords:		"Acetylcysteine/*chemistry/*urine Biomarkers/urine Chromatography, Liquid/*methods Humans Limit of Detection Linear Models Male Occupational Exposure/*analysis Reproducibility of Results Tandem Mass Spectrometry/*methods Volatile Organic Compounds/toxicity;"
Notes:		"MedlineFrigerio, Gianfranco Mercadante, Rosa Polledri, Elisa Missineo, Pasquale Campo, Laura Fustinoni, Silvia eng Netherlands 2019/04/21 J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jun 1; 1117:66-76. doi: 10.1016/j.jchromb.2019.04.015. Epub 2019 Apr 6"