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« Previous Abstract"An alpha-specific gene, SAG1 is required for sexual agglutination in Saccharomyces cerevisiae"    Next AbstractInduction of sexual agglutinability by unsaturated fatty acids in Saccharomyces cerevisiae »

Arch Microbiol


Title:"Chloroquine, a lysosomotropic agent, inhibits zygote formation in yeast"
Author(s):Doi S; Tanabe K; Watanabe M; Yoshimura M;
Address:"Department of Legal Medicine, Kinki University School of Medicine, Osaka, Japan"
Journal Title:Arch Microbiol
Year:1989
Volume:151
Issue:1
Page Number:20 - 25
DOI: 10.1007/BF00444663
ISSN/ISBN:0302-8933 (Print) 0302-8933 (Linking)
Abstract:"Haploid cells of opposite mating type of Saccharomyces cerevisiae conjugate to form zygote. During the conjugation process, the degradation or reorganization of the cell wall and the fusion of the two plasma membranes take place. Since chloroquine inhibits cellular events associated with the reorganization of the plasma membrane, the effect of the drug on conjugation was studied. Chloroquine at a concentration, at which cell growth was not retarded, inhibited zygote formation, while it did not affect other mating functions, such as sexual agglutination, production of and response to mating pheromone. Cells in a mating culture containing chloroquine formed no 'prezygote' suggesting that they were not prepared for entering into fusion process. The inhibitory effect of chloroquine was reversible as cells formed zygote when they were washed after treatment with chloroquine. Zygote formation was unaffected in cells possessing chloroquine within vacuoles after incubation with the drug in complete medium (YPD) at pH 7.5, followed by washing. This suggests that chloroquine inhibits zygote formation by adsorbing to the plasma membrane of S. cerevisiae"
Keywords:"Adsorption Cell Membrane/drug effects/metabolism Chloroquine/pharmacokinetics/*pharmacology Conjugation, Genetic/drug effects Hydrogen-Ion Concentration Saccharomyces cerevisiae/*drug effects/genetics/growth & development Zygote/drug effects;"
Notes:"MedlineDoi, S Tanabe, K Watanabe, M Yoshimura, M eng Germany 1989/01/01 Arch Microbiol. 1989; 151(1):20-5. doi: 10.1007/BF00444663"

 
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