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EMBO J

Title:		Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterization of M-factor and isolation and analysis of two genes encoding the pheromone
Author(s):		Davey J;
Address:		"School of Biochemistry, University of Birmingham, UK"
Journal Title:		EMBO J
Year:		1992
Volume:		11
Issue:		3
Page Number:		951 - 960
DOI:		10.1002/j.1460-2075.1992.tb05134.x
ISSN/ISBN:		0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking)
Abstract:		"Conjugation in the fission yeast Schizosaccharomyces pombe is controlled by the action of mating pheromones. Here I describe the isolation and characterization of M-factor, the pheromone released by M-type cells. M-factor is a nanopeptide in which the carboxy-terminal cysteine residue is carboxy-methylated and S-alkylated, probably with a farnesyl residue: Tyr-Thr-Pro-Lys-Val-Pro-Tyr-Met-Cys(S-farnesyl)-OCH3. Evidence for this structure was obtained by amino acid analysis, mass spectrometry and tandem mass spectrometry of the native M-factor. Two genes encoding the M-factor were also identified and characterized. It appears that M-factor is synthesized as a larger precursor which is post-translationally cleaved and modified to yield the active pheromone. The proposed modifications are consistent with mechanisms known to exist in other yeast and higher eukaryotes"
Keywords:		"Amino Acid Sequence Base Sequence Chromatography, Gel DNA, Fungal/genetics DNA-Binding Proteins Genes, Fungal Intercellular Signaling Peptides and Proteins Molecular Sequence Data Oxidation-Reduction Peptides/chemistry/genetics/*isolation & purification P;"
Notes:		"MedlineDavey, J eng England 1992/03/01 EMBO J. 1992 Mar; 11(3):951-60. doi: 10.1002/j.1460-2075.1992.tb05134.x"