« Previous AbstractUniform formation of uranium oxide nanocrystals inside ordered mesoporous hosts and their potential applications as oxidative catalysts    Next AbstractReconstitution of the S. aureus agr quorum sensing pathway reveals a direct role for the integral membrane protease MroQ in pheromone biosynthesis »

Zhongguo Zhong Yao Za Zhi

Title:		"[Quantitative determination of seven major absorbed volatile constituents in mice brain, liver and blood after intragastric administration of Asari Radix et Rhizoma suspension by headspace-solid phase microextraction-gas chromatography-mass spectrometry]"
Author(s):		Zhang ZW; Liu GX; Xie DM; Tian F; Jia YK; Xu F; Shang MY; Wang X; Cai SQ;
Address:		"Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China"
Journal Title:		Zhongguo Zhong Yao Za Zhi
Year:		2016
Volume:		41
Issue:		2
Page Number:		285 - 293
DOI:		10.4268/cjcmm20160220
ISSN/ISBN:		1001-5302 (Print) 1001-5302 (Linking)
Abstract:		"A headspace-solid phase microextraction-gas chromatography-mass spectrometry method??OHS-SPME-GC-MS??d was adopted for the quantitative study of 4-allylanisole, methyl eugenol, 2,3,5-trimethoxytoluene, 3,4,5-trimethoxytoluene, sarisan, 3,5-dimethoxytoluene and safrole in mice brain, liver tissues and blood after intragastric administration of Asari Radix et Rhizoma. A VF-WAXms (30 mx0.25 mm, 0.25 mum film thickness) capillary column and SPME fiber coated with 65 mum polydimethylsiloxane/divinylbenzene (PDMS/DVB) were used. The calibration curves of seven volatile constituents were established to validate the method's stability (RSD<15%), repeatability (RSD<9.5%), accuracy (RSD<22%), relative recovery (87.0%-108%) and extraction recovery (74.9%-102%). The validated HS-SPME-GC-MS assay was applied to determine the concentrations of seven constituents in liver, brain and blood. The detected contents were 0.22,0.14 mug*g(-)(1),0.25 mg*L(-)(1) (4-allylanisole), 1.1, 0.39 mug*g(-)(1), 0.69 mg*L(-)(1) (methyl eugenol), 0.45, 0.13 mug*g(-)(1), 0.54 mg*L(-)(1) (2,3,5-trimethoxytoluene), 0.51, 0.15 mug*g(-)(1), 0.45 mg*L(-)(1) (3,4,5-trimethoxytoluene), 0.48, 0.039 mug*g(-)(1), 0.69 mg*L (-)(1) (sarisan), 2.2, 1.2 mug*g(-)(1), 1.5 mg*L(-)(1) (3,5-dimethoxytoluene) and 1.3, 0.67 mug*g(-)(1), 1.1 mg*L(-)(1) (safrole) respectively. This HS-SPME-GC-MS method is rapid and convenient, with a small sample size, and applicable for the analysis and determination of volatile constituents in traditional Chinese medicines, which provides scientific data for further studies on effective substances and toxic substances in Asari Radix et Rhizoma"
Keywords:		"Animals Blood/metabolism *Blood Chemical Analysis Brain/drug effects/*metabolism Brain Chemistry Drugs, Chinese Herbal/*chemistry/isolation & purification Gas Chromatography-Mass Spectrometry/*methods Liver/chemistry/drug effects/*metabolism Male Mice Mic;"
Notes:		"MedlineZhang, Zi-Wei Liu, Guang-Xue Xie, De-Mei Tian, Feng Jia, Yi-Kang Xu, Feng Shang, Ming-Ying Wang, Xuan Cai, Shao-Qing chi Evaluation Study China 2016/01/01 Zhongguo Zhong Yao Za Zhi. 2016 Jan; 41(2):285-293. doi: 10.4268/cjcmm20160220"