| Title: | RNAi-mediated silencing of SlitPer disrupts sex pheromone communication behavior in Spodoptera litura |
| Author(s): | Yang HH; Li JQ; Ma S; Yao WC; Chen YW; El Wakil A; Dewer Y; Zhu XY; Sun L; Zhang YN; |
| Address: | "College of Life Sciences, Huaibei Normal University, Huaibei, China. Biological and Geological Sciences Department, Faculty of Education, Alexandria University, Alexandria, Egypt. Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza, Egypt. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: The 24-h circadian rhythm is considered crucial for insect sexual communication. However, its molecular mechanisms and signaling pathways, particularly the roles of the clock gene period (Per), remain largely unclear. The sex pheromone communication behavior of Spodoptera litura displays typical circadian rhythm characteristics. Thus, it represents an excellent model for functional analyses of the clock gene Per. RESULTS: In this study, we investigated the potential roles of SlitPer in regulating sex pheromone communication in S. litura using RNA interference, quantitative real-time polymerase chain reactions (qPCR), gas chromatography, and behavioral assays. The qPCR results showed that the expression levels of SlitPer and two desaturase genes (SlitDes5 and SlitDes11) in the siPer group differed significantly at most time points from those in the siNC group. Dynamic variation in the three major sex pheromone titers and calling behavior of S. litura females in the siPer group was disordered. In addition, the mating rates of siPer S. litura females decreased significantly by 33.33%. Oviposition by mated siPer females was substantially reduced by 84.84%. CONCLUSION: These findings provide a fundamental basis for elucidating the molecular mechanism by which Per regulates sex pheromone communication behavior in lepidopteran species. (c) 2023 Society of Chemical Industry" |
| Keywords: | RNAi Spodoptera litura circadian rhythm period sex pheromone communication; |
| Notes: | "PublisherYang, Hui-Hui Li, Jian-Qiao Ma, Sai Yao, Wei-Chen Chen, Yu-Wen El Wakil, Abeer Dewer, Youssef Zhu, Xiu-Yun Sun, Liang Zhang, Ya-Nan eng Anhui Provincial Natural Science Foundation/ Central Public-Interest Scientific Institution Basal Research Fund/ National College Students' Innovation and Entrepreneurship Training Program/ National Natural Science Foundation of China/ Natural Science Fund of Education Department of Anhui Province, China/ England 2023/06/03 Pest Manag Sci. 2023 Oct; 79(10):3993-3998. doi: 10.1002/ps.7593. Epub 2023 Jun 16" |
