| Title: | Quantification of seven microbial volatile organic compounds in human serum by solid-phase microextraction gas chromatography-tandem mass spectrometry |
| Author(s): | Wazeerud-Din IJ; Silva LK; Smith MM; Newman CA; Blount BC; De Jesus VR; |
| Address: | "Division of Laboratory Sciences, National Center for Environmental Health, U.S. Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA. Division of Laboratory Sciences, National Center for Environmental Health, U.S. Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA. Electronic address: zox1@cdc.gov" |
| DOI: | 10.1016/j.chemosphere.2020.128970 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "Microbial volatile organic compounds (MVOCs) are primary and secondary metabolites of fungal and bacterial growth. Changes in environmental conditions (e.g., humidity, light, oxygen, and carbon dioxide) influence microbial growth in indoor environments. Prolonged human exposure to MVOCs has been directly associated with sick building syndrome (SBS), respiratory irritation, and asthma-like symptoms. However, no method exists for assessing MVOC exposure by quantifying them in human serum. We developed a novel, high-throughput automated method for quantifying seven MVOCs (3-methylfuran, 2-hexanone, 2-heptanone, 3-octanone, 1-octen-3-ol, 2-ethyl-1-hexanol, and geosmin) in human serum. The method quantifies the target analytes using solid-phase microextraction gas chromatography-tandem mass spectrometry at low parts-per-billion levels. Limits of detection ranged from 0.076 to 2.77 mug/L. This method provides excellent linearity over the concentration range for the analytes, with coefficients of determination >0.992. Recovery in human serum was between 84.5% and 113%, and analyte precision ranged from 0.38% to 8.78%. The intra-day and inter-day reproducibility showed coefficients of variation |
| Keywords: | "*Air Pollution, Indoor/analysis Gas Chromatography-Mass Spectrometry Humans Reproducibility of Results Solid Phase Microextraction Tandem Mass Spectrometry *Volatile Organic Compounds/analysis 1-Octen-3-ol 2-Ethyl-1-hexanol 3-Methylfuran 3-Octanone Geosmi;" |
| Notes: | "MedlineWazeerud-Din, Idris J Silva, Lalith K Smith, Mitchell M Newman, Cody A Blount, Benjamin C De Jesus, Victor R eng England 2020/11/25 Chemosphere. 2021 Mar; 266:128970. doi: 10.1016/j.chemosphere.2020.128970. Epub 2020 Nov 13" |
