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Anal Chem

Title:		Study of Controlled Atmosphere Flexible Microtube Plasma Soft Ionization Mass Spectrometry for Detection of Volatile Organic Compounds as Potential Biomarkers in Saliva for Cancer
Author(s):		Vogel P; Lazarou C; Gazeli O; Brandt S; Franzke J; Moreno-Gonzalez D;
Address:		"ISAS-Leibniz-Institut fur Analytische Wissenschaften, Bunsen Kirchhoff Strasse 11, Dortmund 44139, Germany. FOSS Research Centre for Sustainable Energy, PV Technology, University of Cyprus, 75 Kallipoleos Street, Nicosia 1678, Cyprus"
Journal Title:		Anal Chem
Year:		2020
Volume:		20200709
Issue:		14
Page Number:		9722 - 9729
DOI:		10.1021/acs.analchem.0c01063
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"A new soft ionization device for mass spectrometry is presented using the flexible microtube plasma under controlled atmospheric conditions. The controlled atmosphere flexible microtube plasma consists of the plasma source itself connected to a gas chromatograph and a mass spectrometer using a borosilicate glass cross piece. Controlled atmosphere, for example, nitrogen and/or an oxygen mixture, is introduced to the system to create a clean ionization environment. Reproducibility issues are discussed, and solutions are presented manipulating the gas flow in the cross piece. A proof of concept is shown using a ketone mixture introduced to the mass spectrometer to optimize atmospheric conditions. Furthermore, application of the presented device for the sensitive and nonfragmenting ionization of volatile organic biomarkers relevant for cancer is carried out. Sample treatment for human saliva is described, and relevant candidate biomarkers are measured in the saliva matrix, showing a very good ionization efficiency and neglectable matrix effects with limits of detection below 80 ppt"
Keywords:		"Biomarkers, Tumor/*chemistry Gas Chromatography-Mass Spectrometry Humans Mass Spectrometry/*instrumentation/*methods Neoplasms/*diagnosis Reproducibility of Results Saliva/*chemistry Volatile Organic Compounds/*chemistry;"
Notes:		"MedlineVogel, Pascal Lazarou, Constantinos Gazeli, Odhisea Brandt, Sebastian Franzke, Joachim Moreno-Gonzalez, David eng Research Support, Non-U.S. Gov't 2020/06/25 Anal Chem. 2020 Jul 21; 92(14):9722-9729. doi: 10.1021/acs.analchem.0c01063. Epub 2020 Jul 9"