| Title: | "yam8(+), a Schizosaccharomyces pombe gene, is a potential homologue of the Saccharomyces cerevisiae MID1 gene encoding a stretch-activated Ca(2+)-permeable channel" |
| Author(s): | Tasaka Y; Nakagawa Y; Sato C; Mino M; Uozumi N; Murata N; Muto S; Iida H; |
| Address: | "Research Institute for Biological Sciences, 7549-1 Yoshikawa, Kayo, Joubo, Okayama, 716-1241, Japan" |
| Journal Title: | Biochem Biophys Res Commun |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "The Saccharomyces cerevisiae MID1 gene encodes a stretch-activated Ca(2+)-permeable channel. In a protein database, we found a Schizosaccharomyces pombe gene whose predicted protein shows 26% identical and 62% similar to the Mid1 channel in amino acid sequence. cDNA derived from this gene, designated yam8(+), was isolated by reverse transcription-polymerase chain reaction (RT-PCR). Further analysis showed that the Yam8 protein consists of 486 amino acids and has 6 hydrophobic segments. The yam8(+) cDNA, placed under the S. cerevisiae TDH3 promoter, partially complemented the mating pheromone-induced death (mid) phenotype of the S. cerevisiae mid1 mutant. The expression of the yam8(+) cDNA in the mid1 mutant cells partially remediated the mid phenotype and resulted in a slight increase in Ca(2+) uptake activity. These findings suggest that Yam8 is a potential homologue of Mid1" |
| Keywords: | "Amino Acid Sequence Base Sequence Calcium Channels/chemistry/*genetics DNA Primers/genetics DNA, Complementary/genetics DNA, Fungal/genetics Fungal Proteins/chemistry/*genetics Gene Expression *Genes, Fungal Genetic Complementation Test Membrane Glycoprot;" |
| Notes: | "MedlineTasaka, Y Nakagawa, Y Sato, C Mino, M Uozumi, N Murata, N Muto, S Iida, H eng Comparative Study Research Support, Non-U.S. Gov't 2000/03/01 Biochem Biophys Res Commun. 2000 Mar 5; 269(1):265-9. doi: 10.1006/bbrc.2000.2278" |
