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Anal Chem

Title:		Simultaneous analysis of 22 volatile organic compounds in cigarette smoke using gas sampling bags for high-throughput solid-phase microextraction
Author(s):		Sampson MM; Chambers DM; Pazo DY; Moliere F; Blount BC; Watson CH;
Address:		"Centers for Disease Control and Prevention , 4770 Buford Highway, Atlanta, Georgia 30341, United States"
Journal Title:		Anal Chem
Year:		2014
Volume:		20140703
Issue:		14
Page Number:		7088 - 7095
DOI:		10.1021/ac5015518
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Print) 0003-2700 (Linking)
Abstract:		"Quantifying volatile organic compounds (VOCs) in cigarette smoke is necessary to establish smoke-related exposure estimates and evaluate emerging products and potential reduced-exposure products. In response to this need, we developed an automated, multi-VOC quantification method for machine-generated, mainstream cigarette smoke using solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS). This method was developed to simultaneously quantify a broad range of smoke VOCs (i.e., carbonyls and volatiles, which historically have been measured by separate assays) for large exposure assessment studies. Our approach collects and maintains vapor-phase smoke in a gas sampling bag, where it is homogenized with isotopically labeled analogue internal standards and sampled using gas-phase SPME. High throughput is achieved by SPME automation using a CTC Analytics platform and custom bag tray. This method has successfully quantified 22 structurally diverse VOCs (e.g., benzene and associated monoaromatics, aldehydes and ketones, furans, acrylonitrile, 1,3-butadiene, vinyl chloride, and nitromethane) in the microgram range in mainstream smoke from 1R5F and 3R4F research cigarettes smoked under ISO (Cambridge Filter or FTC) and Intense (Health Canada or Canadian Intense) conditions. Our results are comparable to previous studies with few exceptions. Method accuracy was evaluated with third-party reference samples (
Keywords:		Automation Equipment Design Gas Chromatography-Mass Spectrometry/*methods Quality Control Smoke/*analysis Solid Phase Microextraction/instrumentation/*methods/standards Tobacco *Tobacco Products Volatile Organic Compounds/*analysis;
Notes:		"MedlineSampson, Maureen M Chambers, David M Pazo, Daniel Y Moliere, Fallon Blount, Benjamin C Watson, Clifford H eng CC999999/Intramural CDC HHS/ Research Support, U.S. Gov't, P.H.S. 2014/06/17 Anal Chem. 2014 Jul 15; 86(14):7088-95. doi: 10.1021/ac5015518. Epub 2014 Jul 3"