| Title: | Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast |
| Author(s): | Pozniakovsky AI; Knorre DA; Markova OV; Hyman AA; Skulachev VP; Severin FF; |
| Address: | "Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany" |
| ISSN/ISBN: | 0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking) |
| Abstract: | "Although programmed cell death (PCD) is extensively studied in multicellular organisms, in recent years it has been shown that a unicellular organism, yeast Saccharomyces cerevisiae, also possesses death program(s). In particular, we have found that a high doses of yeast pheromone is a natural stimulus inducing PCD. Here, we show that the death cascades triggered by pheromone and by a drug amiodarone are very similar. We focused on the role of mitochondria during the pheromone/amiodarone-induced PCD. For the first time, a functional chain of the mitochondria-related events required for a particular case of yeast PCD has been revealed: an enhancement of mitochondrial respiration and of its energy coupling, a strong increase of mitochondrial membrane potential, both events triggered by the rise of cytoplasmic [Ca2+], a burst in generation of reactive oxygen species in center o of the respiratory chain complex III, mitochondrial thread-grain transition, and cytochrome c release from mitochondria. A novel mitochondrial protein required for thread-grain transition is identified" |
| Keywords: | Amiodarone/*pharmacology Antifungal Agents/pharmacology Antimycin A/pharmacology Apoptosis/drug effects/*physiology Calcium/metabolism Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology Cytochromes c/metabolism DNA Fragmentation/drug effects; |
| Notes: | "MedlinePozniakovsky, Andrei I Knorre, Dmitry A Markova, Olga V Hyman, Anthony A Skulachev, Vladimir P Severin, Fedor F eng Research Support, Non-U.S. Gov't 2005/01/20 J Cell Biol. 2005 Jan 17; 168(2):257-69. doi: 10.1083/jcb.200408145" |
