« Previous Abstract"Relative congener scaling of Polychlorinated dibenzo-p-dioxins and dibenzofurans to estimate building fire contributions in air, surface wipes, and dust samples"    Next AbstractUsing the US EPA CompTox Chemicals Dashboard to interpret targeted and non-targeted GC-MS analyses from human breath and other biological media »

J Breath Res

Title:		Volatile polar metabolites in exhaled breath condensate (EBC): collection and analysis
Author(s):		Pleil JD; Hubbard HF; Sobus JR; Sawyer K; Madden MC;
Address:		"Human Exposure and Atmospheric Sciences Division, National Exposure Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA"
Journal Title:		J Breath Res
Year:		2008
Volume:		20080110
Issue:		2
Page Number:		26001 -
DOI:		10.1088/1752-7155/2/2/026001
ISSN/ISBN:		1752-7155 (Print) 1752-7155 (Linking)
Abstract:		"Environmental exposures, individual activities and disease states can perturb normal metabolic processes and be expressed as a change in the patterns of polar volatile organic compounds (PVOCs) present in biological fluids. We explore the measurement of volatile endogenous biomarkers to infer previous exposures to complex mixtures of environmental stressors. It is difficult to extract such compounds for ultra-trace level analysis due to their high solubility in water, especially when assaying complex liquid biological media such as exhaled breath condensate (EBC). Existing methods tend to be limited in sample volume processed and restricted in sample throughput. We have developed an alternative passive extraction method wherein a 2 ml sample is injected into a 75 ml glass bulb creating a small pool of liquid; a standard Tenax(R) sampling tube is inserted above the fluid and allowed to equilibrate with the headspace for approximately 24 h. The biomarker compounds are preferentially transferred by diffusion from the aqueous sample onto the Tenax(R) adsorbent; blanks and calibration samples are similarly processed. Numerous samples can be simultaneously prepared and stored awaiting routine analysis for a suite of alcohols and aldehydes using thermal desorption gas chromatography-mass spectrometry (GC-MS). We have optimized the procedures and estimated the sensitivity, precision and extraction efficiency resulting from the preparation and analytical procedures using synthetic samples. We subsequently demonstrated the method using anonymous biological specimens of EBC from healthy adults. The ultimate goal is to develop normal ranges and patterns for PVOCs to infer population-based environmental health states with simple spot measurements based on outlier determinations"
Keywords:
Notes:		"PubMed-not-MEDLINEPleil, Joachim D Hubbard, Heidi F Sobus, Jon R Sawyer, Keegan Madden, Michael C eng England 2008/06/01 J Breath Res. 2008 Jun; 2(2):026001. doi: 10.1088/1752-7155/2/2/026001. Epub 2008 Jan 10"