| Title: | Role of the Saccharomyces cerevisiae Rad51 paralogs in sister chromatid recombination |
| Author(s): | Mozlin AM; Fung CW; Symington LS; |
| Address: | "Department of Microbiology, Columbia University Medical Center, New York, New York 10032, USA" |
| DOI: | 10.1534/genetics.107.082677 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "Rad51 requires a number of other proteins, including the Rad51 paralogs, for efficient recombination in vivo. Current evidence suggests that the yeast Rad51 paralogs, Rad55 and Rad57, are important in formation or stabilization of the Rad51 nucleoprotein filament. To gain further insights into the function of the Rad51 paralogs, reporters were designed to measure spontaneous or double-strand break (DSB)-induced sister or nonsister recombination. Spontaneous sister chromatid recombination (SCR) was reduced 6000-fold in the rad57 mutant, significantly more than in the rad51 mutant. Although the DSB-induced recombination defect of rad57 was suppressed by overexpression of Rad51, elevated temperature, or expression of both mating-type alleles, the rad57 defect in spontaneous SCR was not strongly suppressed by these same factors. In addition, the UV sensitivity of the rad57 mutant was not strongly suppressed by MAT heterozygosity, even though Rad51 foci were restored under these conditions. This lack of suppression suggests that Rad55 and Rad57 have different roles in the recombinational repair of stalled replication forks compared with DSB repair. Furthermore, these data suggest that most spontaneous SCR initiates from single-stranded gaps formed at stalled replication forks rather than DSBs" |
| Keywords: | "Adenosine Triphosphatases DNA Breaks, Double-Stranded/drug effects/radiation effects DNA Breaks, Single-Stranded/drug effects/radiation effects DNA Repair Enzymes DNA-Binding Proteins/*metabolism Diploidy Gene Conversion/drug effects/radiation effects Het;" |
| Notes: | "MedlineMozlin, Amy M Fung, Cindy W Symington, Lorraine S eng R01 GM054099/GM/NIGMS NIH HHS/ R01 GM054099-12/GM/NIGMS NIH HHS/ R01 GM054099-11/GM/NIGMS NIH HHS/ T32 AI07161/AI/NIAID NIH HHS/ T32 GM08224/GM/NIGMS NIH HHS/ T32 GM008224/GM/NIGMS NIH HHS/ T32 AI007161/AI/NIAID NIH HHS/ GM054099/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2008/01/19 Genetics. 2008 Jan; 178(1):113-26. doi: 10.1534/genetics.107.082677" |
