| Title: | Proteomic and transcriptomic changes in rat liver following oral feeding of formaldehyde |
| Author(s): | Mohanty BP; Mahanty A; Mitra T; Mohanty S; Naik AK; Parija SC; |
| Address: | "ICAR-Central Inland Fisheries Research Institute, FREM Division, Biochemistry Laboratory- Proteomics Unit, Barrackpore, Kolkata, 700120, West Bengal, India. Electronic address: bimal.mohanty@icar.gov.in. ICAR-Central Inland Fisheries Research Institute, FREM Division, Biochemistry Laboratory- Proteomics Unit, Barrackpore, Kolkata, 700120, West Bengal, India; ICAR-National Rice Research Institute, Crop Protection Division, Cuttack, 753006, India. ICAR-Central Inland Fisheries Research Institute, FREM Division, Biochemistry Laboratory- Proteomics Unit, Barrackpore, Kolkata, 700120, West Bengal, India. Department of Biotechnology, Faculty of Science and Technology, Rama Devi Women's' University, Bhubabeswar, 751022, India. Department of Pharmacology & Toxicology, Faculty of Veterinary Science, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India" |
| DOI: | 10.1016/j.chemosphere.2019.125599 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "Formaldehyde (FA), a ubiquitous volatile organic compound present in a wide range of resources, is a hazardous chemical and human carcinogen. Contamination of FA in food, especially perishable commodities like fish and meat, is a major source of exposure, although it is not recommended for use in food and food products owing to its carcinogenicity. Effects of oral feeding of FA have been studied by evaluating general health, haematology and clinical chemistry in rat. Recent studies have shown that FA exposure leads to detrimental cardiovascular effects. It regulates vascular tensions through nitric oxide-cGMP signalling pathway and ion channels in rats. Although FA is an established carcinogen, molecular studies on carcinogenic potential with dose dependency are meagre. In this context, the present study was undertaken to investigate the toxicogenomic and proteomic alterations in liver of rats fed FA through drinking water. By proteomic analysis, 621 proteins/protein-subunits showed differential abundance (proteome data available via ProteomeXchange with identifier PXD010534), whereas 536 differentially-expressed-genes were identified by transcriptome analysis (data available via Sequence Read Archive with identifier SRR7974113). Gene ontology analysis showed that binding, catalysis, signal transduction were affected in formaldehyde-fed rats. Pathway analysis revealed that formaldehyde-exposure activated PI3K-AKT pathway that leads to inhibition of caspase activity thereby assisting cells to survive against apoptosis. Decreased abundance/down-regulation of ANGPT, eNOS, STAT3 proteins/transcripts and increased abundance of EDN1 indicated decrease in angiogenesis and vasodilatation that restricted hepatic cells from becoming tumorigenic; thus, indicating FA could be less toxic and non-tumorigenic at low concentrations" |
| Keywords: | Animals Apoptosis/drug effects Formaldehyde/*pharmacology/toxicity Gene Expression Regulation/drug effects Humans Liver/drug effects/pathology Phosphatidylinositol 3-Kinases/metabolism Proteome/*drug effects Rats Signal Transduction/drug effects Transcrip; |
| Notes: | "MedlineMohanty, Bimal Prasanna Mahanty, Arabinda Mitra, Tandrima Mohanty, Sasmita Naik, Ajit Kumar Parija, Subas Chandra eng England 2019/12/20 Chemosphere. 2020 Apr; 245:125599. doi: 10.1016/j.chemosphere.2019.125599. Epub 2019 Dec 9" |
