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Mol Cell Biol

Title:		Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones
Author(s):		Chan RK; Otte CA;
Address:
Journal Title:		Mol Cell Biol
Year:		1982
Volume:		2
Issue:		1
Page Number:		11 - 20
DOI:		10.1128/mcb.2.1.11-20.1982
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Eight independently isolated mutants which are supersensitive (Sst-) to the G1 arrest induced by the tridecapeptide pheromone alpha factor were identified by screening mutagenized Saccharomyces cerevisiae MATa cells on solid medium for increased growth inhibition by alpha factor. These mutants carried lesions in two complementation groups, sst1 and sst2. Mutations at the sst1 locus were mating type specific: MATa sst1 cells were supersensitive to alpha factor, but MAT alpha sst1 cells were not supersensitive to a factor. In contrast, mutations at the sst2 locus conferred supersensitivity to the pheromones of the opposite mating type on both MATa and MAT alpha cells. Even in the absence of added alpha pheromone, about 10% of the cells in exponentially growing cultures of MATa strains carrying any of three different alleles of sst2 (including the ochre mutation sst2-4) had the aberrant morphology ('shmoo' shape) that normally develops only after MATa cells are exposed to alpha factor. This 'self-shmooing' phenotype was genetically linked to the sst2 mutations, although the leakiest allele isolated (sst2-3) did not display this characteristic. Normal MATa/MAT alpha diploids do not respond to pheromones; diploids homozygous for an sst2 mutation (MATa/MAT alpha sst2-1/sst2-1) were still insensitive to alpha factor. The sst1 gene was mapped to within 6.9 centimorgans of his6 on chromosome IX. The sst2 gene was unlinked to sst1, was not centromere linked, and was shown to be neither linked to nor centromere distal to MAT on the right arm of chromosome III"
Keywords:		"Alleles Chromosome Mapping Crosses, Genetic Fungal Proteins/*pharmacology Genetic Linkage Interphase/*drug effects Mating Factor Mutation Peptides/*pharmacology Phenotype Saccharomyces cerevisiae/*genetics;"
Notes:		"MedlineChan, R K Otte, C A eng Research Support, Non-U.S. Gov't 1982/01/01 Mol Cell Biol. 1982 Jan; 2(1):11-20. doi: 10.1128/mcb.2.1.11-20.1982"