| Title: | RAP1 protein activates and silences transcription of mating-type genes in yeast |
| Address: | "Department of Microbiology, College of Physicians & Surgeons, Columbia University, New York, New York 10032" |
| ISSN/ISBN: | 0890-9369 (Print) 0890-9369 (Linking) |
| Abstract: | "RAP1 is a sequence-specific DNA-binding protein essential for cell growth. The occurrence of RAP1-binding sites in many promoter regions, the mating-type gene silencer elements, and telomeres suggests that RAP1 has multiple functions in the cell. To assess its role in transcription, temperature-sensitive mutations in RAP1 were generated. Analysis of rap1ts strains provides evidence that RAP1 functions in both transcriptional activation and silencing of mating-type genes. Several observations indicate that rap1ts strains are defective in the expression of MAT alpha, whose upstream activation sequence (UAS) contains a RAP1-binding site. At nonpermissive temperatures, decreases in MAT alpha steady-state transcript levels can be detected in MAT alpha rap1ts strains. Furthermore, these strains are deficient in alpha-pheromone production and simultaneously express at least two alpha-specific genes. These phenotypes can be reversed by replacing the RAP1-binding site at MAT alpha with a binding site for the GAL4 transcriptional activator. Certain rap1ts alleles have an opposite effect on the silent mating-type locus HMR, which becomes partially derepressed at nonpermissive temperatures" |
| Keywords: | "Alleles Crosses, Genetic DNA-Binding Proteins/*metabolism Fungal Proteins/*metabolism *Gene Expression Regulation, Fungal *Genes, Fungal *Genes, Mating Type, Fungal Genotype Kinetics Mating Factor Peptides/*genetics Pheromones/metabolism Promoter Regions, ;" |
| Notes: | "MedlineKurtz, S Shore, D eng GM40094/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1991/04/01 Genes Dev. 1991 Apr; 5(4):616-28. doi: 10.1101/gad.5.4.616" |
