| Title: | Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells |
| Author(s): | Kuchler K; Sterne RE; Thorner J; |
| Address: | "Department of Biochemistry, University of California, Berkeley 94720" |
| DOI: | 10.1002/j.1460-2075.1989.tb08580.x |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "Saccharomyces cerevisiae MATa cells release a lipopeptide mating pheromone, a-factor. Radiolabeling and immunoprecipitation show that MATa ste6 mutants produce pro-a-factor and mature a-factor intracellularly, but little or no extracellular pheromone. Normal MATa cells carrying a multicopy plasmid containing both MFa1 (pro-a-factor structural gene) and the STE6 gene secrete a-factor at least five times faster than the same cells carrying only MFa1 in the same vector. The nucleotide sequence of the STE6 gene predicts a 1290 residue polypeptide with multiple membrane spanning segments and two hydrophilic domains, each strikingly homologous to a set of well-characterized prokaryotic permeases (including hlyB, oppD, hisP, malK and pstB) and sharing even greater identity with mammalian mdr (multiple drug resistance) transporters. These results suggest that the STE6 protein in yeast, and possibly mdr in animals, is a transmembrane translocator that exports polypeptides by a route independent of the classical secretory pathway" |
| Keywords: | "*ATP-Binding Cassette Transporters Amino Acid Sequence Animals Base Sequence Fungal Proteins/biosynthesis/*genetics *Genes, Fungal Genotype *Glycoproteins Kinetics Molecular Sequence Data Mutation Pheromones/*genetics Protein Precursors/*genetics *Protein;" |
| Notes: | "MedlineKuchler, K Sterne, R E Thorner, J eng CA09041/CA/NCI NIH HHS/ GM07232/GM/NIGMS NIH HHS/ GM21841/GM/NIGMS NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1989/12/20 EMBO J. 1989 Dec 20; 8(13):3973-84. doi: 10.1002/j.1460-2075.1989.tb08580.x" |
