| Title: | "Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata" |
| Author(s): | Keinanen M; Oldham NJ; Baldwin IT; |
| Address: | "Department of Molecular Ecology, Max-Planck Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany" |
| ISSN/ISBN: | 0021-8561 (Print) 0021-8561 (Linking) |
| Abstract: | "A rapid, HPLC-based screening procedure for the main classes of secondary metabolites in Nicotiana attenuata leaves (alkaloids, phenolics, and diterpene glycosides) is reported. In a single step, leaves are extracted in aqueous acidified (0.5% acetic acid) methanol, and the extracted compounds are separated by reversed-phase HPLC with an acidic water/acetonitrile gradient in <30 min. The utility of the method in quantifying changes in the secondary metabolites after methyl jasmonate treatment of the plants, a treatment known to elicit resistance to herbivores in nature, is illustrated. Methyl jasmonate treatment elicited dramatic increases in some secondary metabolites (caffeoylputrescine, nicotine, and diterpene glycosides increased 12.5-, 1.4-, and 1.9-fold, respectively) but left others, such as rutin, unchanged. Such broad-based analytical screens will help characterize environmental and genetic changes in secondary metabolite profiles" |
| Keywords: | "Alkaloids/metabolism Chromatography, High Pressure Liquid/*methods Cyclopentanes/pharmacology Diterpenes/metabolism Oxylipins Phenols/metabolism Plant Leaves/chemistry *Plants, Toxic Tobacco/*chemistry/*metabolism;" |
| Notes: | "MedlineKeinanen, M Oldham, N J Baldwin, I T eng 2001/08/22 J Agric Food Chem. 2001 Aug; 49(8):3553-8. doi: 10.1021/jf010200+" |
