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J Chem Ecol

Title:		"Binding characterization of recombinant odorant-binding proteins from the parasitic wasp, Microplitis mediator (Hymenoptera: Braconidae)"
Author(s):		Zhang S; Chen LZ; Gu SH; Cui JJ; Gao XW; Zhang YJ; Guo YY;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China"
Journal Title:		J Chem Ecol
Year:		2011
Volume:		20101224
Issue:		2
Page Number:		189 - 194
DOI:		10.1007/s10886-010-9902-3
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"Chemoreception in insects is mediated by small odorant-binding proteins (OBPs) that are believed to carry lipophilic stimuli to the olfactory receptor cells through the aqueous sensillar lymph. Binding experiments and recent structural studies of OBPs have illustrated their versatility and ability to accommodate ligands of different shapes and chemical structures. We expressed and purified seven recombinant OBPs (MmedOBP1-MmedOBP7) from the parasitic wasp, Microplitis mediator (Hymenoptera: Braconidae) in a prokaryotic expression system. With 4,4'-dianilino-1,1'-binaphthyl-5,5'-sulfonic acid (bis-ANS) as a fluorescent probe, the ligand-binding specificities of these seven MmedOBPs with 50 small organic compounds were investigated in vitro. The results revealed that all of the M. mediator OBPs can bind a wide variety of odorant molecules with different binding affinities. The best ligand for all seven MmedOBPs was beta-ionone. MmedOBP2 showed affinity for some aromatic compounds, whereas MmedOBP4 and MmedOBP6 bound several terpenoids. MmedOBP5 bound beta-ionone, but did not bind any of the other potential ligands that we tested"
Keywords:		"Anilino Naphthalenesulfonates/chemistry/metabolism Animals Female Fluorescent Dyes/chemistry/metabolism *Hymenoptera Insect Proteins/*metabolism Male Plants/chemistry Protein Binding Receptors, Odorant/*metabolism Recombinant Proteins/*metabolism Substrat;"
Notes:		"MedlineZhang, Shuai Chen, Li-Zhen Gu, Shao-Hua Cui, Jin-Jie Gao, Xi-Wu Zhang, Yong-Jun Guo, Yu-Yuan eng Research Support, Non-U.S. Gov't 2010/12/25 J Chem Ecol. 2011 Feb; 37(2):189-94. doi: 10.1007/s10886-010-9902-3. Epub 2010 Dec 24"