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Zhongguo Zhong Yao Za Zhi

Title:		[Gene cloning and bioinformatics analysis of SABATH methyltransferase in Lonicera japonica var. chinensis]
Author(s):		Yu XD; Jiang C; Huang LQ; Qin SS; Zeng XM; Chen P; Yuan Y;
Address:		"Wuhan Polytechnic University, Wuhan 430023, China"
Journal Title:		Zhongguo Zhong Yao Za Zhi
Year:		2013
Volume:		38
Issue:		16
Page Number:		2556 - 2562
DOI:
ISSN/ISBN:		1001-5302 (Print) 1001-5302 (Linking)
Abstract:		"OBJECTIVE: To clone SABATH methyltransferase (rLjSABATHMT) gene in Lonicera japonica var. chinensis, and compare the gene expression and intron sequence of SABATH methyltransferase orthologous in L. japonica with L. japonica var. chinensis. It provide a basis for gene regulate the formation of L. japonica floral scents. METHOD: The cDNA and genome sequences of LjSABATHMT from L. japonica var. chinensis were cloned according to the gene fragments in cDNA library. The LjSABATHMT protein was characterized by bioinformatics analysis. SABATH family phylogenetic tree were built by MEGA 5.0. The transcripted level of SABATHMT orthologous were analyzed in different organs and different flower periods of L. japonica and L. japonica var. chinensis using RT-PCR analysis. Intron sequences of SABATHMT orthologous were also analyzied. RESULT: The cDNA of LjSABATHMT was 1 251 bp, had a complete coding frame with 365 amino acids. The protein had the conservative SABATHMT domain, and phylogenetic tree showed that it may be a salicylic acid/benzoic acid methyltransferase. Higher expression of SABATH methyltransferase orthologous was found in flower. The intron sequence of L. japonica and L. japonica var. chinensis had rich polymorphism, and two SNP are unique genotype of L. japonica var. chinensis. The motif elements in two orthologous genes were significant differences. CONCLUSION: The intron difference of SABATH methyltransferase orthologous could be inducing to difference of gene expression between L. japonica and L. japonica var. chinensis. These results will provide important base on regulating active compounds of L. japonica"
Keywords:		"Base Sequence Cloning, Molecular *Computational Biology Evolution, Molecular Gene Expression Regulation, Plant Lonicera/*enzymology/*genetics Methyltransferases/*genetics Nucleotide Motifs Phylogeny Sequence Alignment Sequence Analysis;"
Notes:		"MedlineYu, Xiao-Dan Jiang, Chao Huang, Lu-Qi Qin, Shuang-Shuang Zeng, Xiang-Mei Chen, Ping Yuan, Yuan chi English Abstract China 2013/11/16 Zhongguo Zhong Yao Za Zhi. 2013 Aug; 38(16):2556-62"