| Title: | Microbial volatile compounds-induced cytotoxicity in the yeast Saccharomyces cerevisiae: The role of MAPK signaling and proteasome regulatory pathway |
| Author(s): | Wu PH; Ho YL; Ho TS; Chang CH; Ye JC; Wang CH; Sung HM; Huang HJ; Liu CC; |
| Address: | "Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan. Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Bachelor's Degree Program for Indigenous Peoples in Senior Health and Care Management, National Taitung University, Taitung, Taiwan; Master Program in Biomedical Science, National Taitung University, Taitung, Taiwan. Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan; Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan. Electronic address: haojen@mail.ncku.edu.tw. Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address: liucc@mail.ncku.edu.tw" |
| DOI: | 10.1016/j.chemosphere.2019.05.293 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "Microbial volatile organic compounds (mVCs) are formed in the metabolism of microorganisms and widely distributed in nature and pose threats to human health. However, the air pollution by microorganisms is a situation which is poorly understood. In this study, the cytotoxicity of E. aerogenes VCs was evaluated in the model organism Saccharomyces cerevisiae. E. aerogenes VCs inhibited the survival of yeast and triggered the formation of intracellular reactive oxygen species (ROS). The hypersensitive of MAP kinase mpk1/slt2 and 19S regulatory assembly chaperone adc17 mutants to the E. aerogenes VCs indicated cell wall integrity (CWI) pathway together with stress-inducible proteasome assembly regulation are essentially involved in mVCs tolerance mechanism. Furthermore, exposure to the mVCs resulted in the transcriptional upregulation of the CWI pathway, the regulatory particle assembly chaperones, and genes involved in proteasome regulations. Our research suggested that the ROS/MAPK signaling and proteasome regulatory pathway play pivotal roles in the integration and fine-tuning of the mVCs stress response. This study provides a molecular framework for future study of the effects of mVCs on more complex organisms, such as humans" |
| Keywords: | Air Pollutants/analysis Air Pollution/analysis Cell Wall/metabolism Cytoplasm/metabolism Enterobacter aerogenes/*metabolism MAP Kinase Signaling System/*drug effects Mitogen-Activated Protein Kinases/*metabolism Molecular Chaperones/metabolism Proteasome; |
| Notes: | "MedlineWu, Pei-Hsuan Ho, Yueh-Lin Ho, Tzong-Shiann Chang, Ching-Han Ye, Je-Chiuan Wang, Ching-Han Sung, Huang-Mo Huang, Hao-Jen Liu, Ching-Chuan eng England 2019/07/26 Chemosphere. 2019 Oct; 233:786-795. doi: 10.1016/j.chemosphere.2019.05.293. Epub 2019 May 31" |
