| Title: | Multivariate analysis of mainstream tobacco smoke particulate phase by headspace solid-phase micro extraction coupled with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry |
| Author(s): | Brokl M; Bishop L; Wright CG; Liu C; McAdam K; Focant JF; |
| Address: | "CART - Chemistry Department, Organic & Biological Analytical Chemistry, University of Liege, Liege, Belgium. Group Research and Development, British American Tobacco, Regents Park Road, Southampton SO15 8TL, UK. CART - Chemistry Department, Organic & Biological Analytical Chemistry, University of Liege, Liege, Belgium. Electronic address: JF.Focant@ulg.ac.be" |
| DOI: | 10.1016/j.chroma.2014.10.057 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "A method involving headspace solid-phase microextraction (HS-SPME) and comprehensive two-dimensional gas chromatography (GCxGC) coupled to time-of-flight mass spectrometry (TOFMS) was developed and applied to evaluate profiles of volatile compounds present in mainstream tobacco smoke particulate matter trapped on glass fiber filters. Six SPME fibers were tested for the extraction capacities toward selected compounds, showing the best results for the polyacrylate fiber. The optimization of the extraction conditions was carried out using multivariate response surface methodology. Two cigarette types differing in a filter design were analyzed using optimized conditions. A template was built in order to generate comprehensive chemical information, which conceded obtaining consistent information across 24 chromatograms. Principal component analysis (PCA) allowed a clear differentiation of the studied cigarette types. Fisher ratio analysis allowed identification of compounds responsible for the chemical differences between the cigarette samples. Of the selected 143 most important ones, 134 analytes were reduced by the active carbon filter, while for nine, classical cellulose acetate filter was more efficient" |
| Keywords: | Gas Chromatography-Mass Spectrometry/*methods Multivariate Analysis Particulate Matter/*analysis Smoke/*analysis Solid Phase Microextraction/*methods Temperature Tobacco/*chemistry Central composite design (CCD) Comprehensive two-dimensional gas chromatog; |
| Notes: | "MedlineBrokl, Michal Bishop, Louise Wright, Christopher G Liu, Chuan McAdam, Kevin Focant, Jean-Francois eng Netherlands 2014/12/03 J Chromatogr A. 2014 Nov 28; 1370:216-29. doi: 10.1016/j.chroma.2014.10.057. Epub 2014 Oct 24" |
