| Title: | The mating-type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes |
| Author(s): | Wendland J; Vaillancourt LJ; Hegner J; Lengeler KB; Laddison KJ; Specht CA; Raper CA; Kothe E; |
| Address: | "Philipps-Universitat, Fachbereich Biologie-Molekulargenetik, Marburg, Germany" |
| DOI: | 10.1002/j.1460-2075.1995.tb00211.x |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "Analysis of the multispecific B alpha mating-type locus of Schizophyllum commune provided evidence that pheromones and pheromone receptors govern recognition of self versus non-self and sexual development in this homobasidiomycetous fungus. Four subclones of an 8.2 kb genomic fragment carrying B alpha 1 specificity induced B-regulated sexual morphogenesis when introduced into a strain with one of the eight compatible B alpha specificities that are known to exist in nature. One of these clones, which activated all other B alpha specificities, contains a gene termed bar1. The predicted protein product of bar1, as well as that of bar2, a homologous gene isolated from a B alpha 2 strain, has significant homology to known fungal pheromone receptor proteins in the rhodopsin-like superfamily of G protein-linked receptors. The other three active B alpha 1 clones were subcloned further to identify the minimal active element in each clone. Every active subclone contains a putative pheromone gene ending in a signal for possible isoprenylation. A message of approximately 600 bp was observed for one of these genes, bap1(1). This paper presents the first evidence for a system of multiple pheromones and pheromone receptors as a basis for multispecific mating types in a fungus" |
| Keywords: | Amino Acid Sequence Base Sequence Chemoreceptor Cells/*physiology Chromosome Mapping Molecular Sequence Data Pheromones/*physiology Schizophyllum/genetics/*physiology Sequence Alignment Sequence Analysis; |
| Notes: | "MedlineWendland, J Vaillancourt, L J Hegner, J Lengeler, K B Laddison, K J Specht, C A Raper, C A Kothe, E eng CA14051/CA/NCI NIH HHS/ GM15292/GM/NIGMS NIH HHS/ GM31318/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. England 1995/11/01 EMBO J. 1995 Nov 1; 14(21):5271-8. doi: 10.1002/j.1460-2075.1995.tb00211.x" |
