| Title: | The deterrent ability of Xenorhabdus nematophila and Photorhabdus laumondii compounds as a potential novel tool for Lobesia botrana (Lepidoptera: Tortricidae) management |
| Author(s): | Vicente-Diez I; Pou A; Campos-Herrera R; |
| Address: | "Instituto de Ciencias de la Vid y del Vino (ICVV, Gobierno de La Rioja, CSIC, Universidad de La Rioja), Finca La GRajera, Ctra. Burgos Km. 6 Salida 13 Lo-20, Logrono 26007, Spain. Instituto de Ciencias de la Vid y del Vino (ICVV, Gobierno de La Rioja, CSIC, Universidad de La Rioja), Finca La GRajera, Ctra. Burgos Km. 6 Salida 13 Lo-20, Logrono 26007, Spain. Electronic address: raquel.campos@icvv.es" |
| DOI: | 10.1016/j.jip.2023.107911 |
| ISSN/ISBN: | 1096-0805 (Electronic) 0022-2011 (Linking) |
| Abstract: | "The grapevine moth, Lobesia botrana (Lepidoptera: Tortricidae), is a critical pest for vineyards and causes significant economic losses in wine-growing areas worldwide. Identifying and developing novel semiochemical cues (e.g. volatile bacterial compounds) which modify the ovipositional and trophic behaviour of L. botrana in vineyard fields could be a novel control alternative in viticulture. Xenorhabdus spp. and Photorhabdus spp. are becoming one of the best-studied bacterial species due to their potential interest in producing toxins and deterrent factors. In this study, we investigated the effect of the deterrent compounds produced by Xenorhabdus nematophila and Photorhabdus laumondii on the ovipositional moth behaviour and the larval feeding preference of L. botrana. Along with the in-vitro bioassays performed, we screened the potential use of 3 d cell-free bacterial supernatants and 3 and 5 d unfiltered bacterial ferments. In addition, we tested two application systems: (i) contact application of the bacterial compounds and (ii) volatile bacterial compounds application. Our findings indicate that the deterrent effectiveness varied with bacterial species, the use of bacterial cell-free supernatants or unfiltered fermentation product, and the culture times. Grapes soaked in the 3 d X. nematophila and P. laumondii ferments had approximately 55% and approximately 95% fewer eggs laid than the control, respectively. Likewise, the volatile compounds emitted by the 5 d P. laumondii fermentations resulted in approximately 100% avoidance of L. botrana ovipositional activity for three days. Furthermore, both bacterial fermentation products have larval feeding deterrent effects ( approximately 65% of the larva chose the control grapes), and they significantly reduced the severity of damage caused by third instar larva in treated grapes. This study provides insightful information about a novel bacteria-based tool which can be used as an eco-friendly and economical alternative in both organic and integrated control of L. botrana in vineyard" |
| Keywords: | Animals *Xenorhabdus *Photorhabdus *Moths Larva *Vitis Deterrent compounds - Lobesia botrana - oviposition cue repellence Photorhabdus laumondii - Vitis vinifera Volatile Organic Compounds - Xenorhabdus nematophila; |
| Notes: | "MedlineVicente-Diez, Ignacio Pou, Alicia Campos-Herrera, Raquel eng Research Support, Non-U.S. Gov't 2023/03/16 J Invertebr Pathol. 2023 Jun; 198:107911. doi: 10.1016/j.jip.2023.107911. Epub 2023 Mar 13" |
