| Title: | The ecological role of bacterial seed endophytes associated with wild cabbage in the United Kingdom |
| Author(s): | Tyc O; Putra R; Gols R; Harvey JA; Garbeva P; |
| Address: | "Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands. Department of Internal Medicine I, Goethe University, University Hospital Frankfurt, Frankfurt, Germany. Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands. Hawkesbury Institute for the Environment, Western Sydney University, Penrith, Australia. Department of Terrestrial Ecology, Netherlands Institute of Ecology, Wageningen, The Netherlands. Department of Ecological Sciences, Section Animal Ecology, VU University Amsterdam, Amsterdam, The Netherlands" |
| ISSN/ISBN: | 2045-8827 (Electronic) 2045-8827 (Linking) |
| Abstract: | "Endophytic bacteria are known for their ability in promoting plant growth and defense against biotic and abiotic stress. However, very little is known about the microbial endophytes living in the spermosphere. Here, we isolated bacteria from the seeds of five different populations of wild cabbage (Brassica oleracea L) that grow within 15 km of each other along the Dorset coast in the UK. The seeds of each plant population contained a unique microbiome. Sequencing of the 16S rRNA genes revealed that these bacteria belong to three different phyla (Actinobacteria, Firmicutes, and Proteobacteria). Isolated endophytic bacteria were grown in monocultures or mixtures and the effects of bacterial volatile organic compounds (VOCs) on the growth and development on B. oleracea and on resistance against a insect herbivore was evaluated. Our results reveal that the VOCs emitted by the endophytic bacteria had a profound effect on plant development but only a minor effect on resistance against an herbivore of B. oleracea. Plants exposed to bacterial VOCs showed faster seed germination and seedling development. Furthermore, seed endophytic bacteria exhibited activity via volatiles against the plant pathogen F. culmorum. Hence, our results illustrate the ecological importance of the bacterial seed microbiome for host plant health and development" |
| Keywords: | Actinobacteria/classification/genetics/isolation & purification Bacteria/classification/genetics/*metabolism Brassica/*growth & development/microbiology Endophytes/classification/genetics/*metabolism Firmicutes/classification/genetics/isolation & purifica; |
| Notes: | "MedlineTyc, Olaf Putra, Rocky Gols, Rieta Harvey, Jeffrey A Garbeva, Paolina eng Research Support, Non-U.S. Gov't England 2019/11/14 Microbiologyopen. 2020 Jan; 9(1):e00954. doi: 10.1002/mbo3.954. Epub 2019 Nov 13" |
