| Title: | Highly effective volatile organic compound dissolving strategy based on mist atomization for odorant biosensors |
| Author(s): | Terutsuki D; Mitsuno H; Sato K; Sakurai T; Mase N; Kanzaki R; |
| Address: | "Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. Electronic address: terutsuki@g.brain.imi.i.u-tokyo.ac.jp. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Hamamatsu, Shizuoka, 432-8561, Japan. Department of Agricultural Innovation for Sustainability, Tokyo University of Agriculture, 1737 Funako, Atsugi-shi, Kanagawa, 243-0034, Japan. Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Hamamatsu, Shizuoka, 432-8561, Japan; Research Institute of Green Science and Technology, Shizuoka University, 3-5-1 Johoku, Hamamatsu, Shizuoka, 432-8561, Japan. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. Electronic address: kanzaki@rcast.u-tokyo.ac.jp" |
| DOI: | 10.1016/j.aca.2020.09.043 |
| ISSN/ISBN: | 1873-4324 (Electronic) 0003-2670 (Linking) |
| Abstract: | "The detection of volatile organic compound (VOC) mixtures is crucial in the medical and security fields. Receptor-based odorant biosensors sensitively and selectively detect odorant molecules in a solution; however, odorant molecules generally exist as VOCs in the air and exhibit poor water solubility. Therefore, techniques that enable the dissolution of poorly water-soluble VOCs using portable systems are essential for practical biosensors' applications. We previously proposed a VOC dissolution method based on water atomization to increase the surface area via the generation of fine bubbles, as a proof-of-concept; however, the system was lab-based (non-mobile) and the dissolution was limited to one VOC. In this study, we established a highly effective VOC dissolution method based on mist atomization that can be used in the field. This new method demonstrated a rapid dissolution potential of a sparsely-soluble VOC mixture with various functional groups in distilled water (DW) within 1 min, without the use of any organic solvents. Calcium imaging revealed that odorant receptor 13a-expressing Sf21 cells (Or13a cells) responded to 1-octen-3-ol in the mixture. Further, we successfully developed a field-deployable prototype vacuum and dissolution system with a simple configuration that efficiently captured and rapidly dissolved airborne 1-octen-3-ol in DW. This study proposes a field-deployable system that is appropriate for solubilizing various airborne odorant molecules and therefore is a practical strategy to use in the context of odorant biosensors" |
| Keywords: | "*Biosensing Techniques Odorants *Receptors, Odorant *Volatile Organic Compounds Fine bubble Insect cell Odorant biosensor Odorant receptor Ultrasonic spray Volatile organic compound;" |
| Notes: | "MedlineTerutsuki, Daigo Mitsuno, Hidefumi Sato, Kohei Sakurai, Takeshi Mase, Nobuyuki Kanzaki, Ryohei eng Netherlands 2020/11/17 Anal Chim Acta. 2020 Dec 1; 1139:178-188. doi: 10.1016/j.aca.2020.09.043. Epub 2020 Sep 28" |
