| Title: | Identification of volatile compounds produced by the bacterium Burkholderia tropica that inhibit the growth of fungal pathogens |
| Author(s): | Tenorio-Salgado S; Tinoco R; Vazquez-Duhalt R; Caballero-Mellado J; Perez-Rueda E; |
| Address: | "Programa de Ecologia Genomica, Centro de Ciencias Genomicas, UNAM, Cuernavaca, Mexico. s.tenorio.salgado@gmail.com" |
| ISSN/ISBN: | 2165-5987 (Electronic) 2165-5979 (Print) 2165-5979 (Linking) |
| Abstract: | "It has been documented that bacteria from the Burkholderia genera produce different kinds of compounds that inhibit plant pathogens, however in Burkholderia tropica, an endophytic diazotrophic and phosphate-solubilizing bacterium isolated from a wide diversity of plants, the capacity to produce antifungal compounds has not been evaluated. In order to expand our knowledge about Burkholderia tropica as a potential biological control agent, we analyzed 15 different strains of this bacterium to evaluate their capacities to inhibit the growth of four phytopathogenic fungi, Colletotrichum gloeosporioides, Fusarium culmorum, Fusarium oxysporum and Sclerotium rolffsi. Diverse analytical techniques, including plant root protection and dish plate growth assays and gas chromatography-mass spectroscopy showed that the fungal growth inhibition was intimately associated with the volatile compounds produced by B. tropica and, in particular, two bacterial strains (MTo293 and TTe203) exhibited the highest radial mycelial growth inhibition. Morphological changes associated with these compounds, such as disruption of fungal hyphae, were identified by using photomicrographic analysis. By using gas chromatography-mass spectroscopy technique, 18 volatile compounds involved in the growth inhibition mechanism were identified, including alpha-pinene and limonene. In addition, we found a high proportion of bacterial strains that produced siderophores during growth with different carbon sources, such as alanine and glutamic acid; however, their roles in the antagonism mechanism remain unclear" |
| Keywords: | Biological Control Agents Burkholderia/*chemistry Colletotrichum/drug effects Fungi/*drug effects Fusarium/drug effects Volatile Organic Compounds/*chemistry/*pharmacology Burkholderia tropica antagonism phytopathogenic fungi volatile compound; |
| Notes: | "MedlineTenorio-Salgado, Silvia Tinoco, Raunel Vazquez-Duhalt, Rafael Caballero-Mellado, Jesus Perez-Rueda, Ernesto eng Research Support, Non-U.S. Gov't 2013/05/18 Bioengineered. 2013 Jul-Aug; 4(4):236-43. doi: 10.4161/bioe.23808. Epub 2013 Jul 2" |
