| Title: | Identification and Characterization of Chemosensory Receptors in the Pheromone Gland-Ovipositor of Spodoptera frugiperda (J. E. Smith) |
| Author(s): | Sun YL; Dong JF; Yang HB; Li DX; Tian CH; |
| Address: | "College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, China. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "Chemoreception by moth ovipositors has long been suggested, but underlying molecular mechanisms are mostly unknown. To reveal such chemosensory systems in the current study, we sequenced and assembled the pheromone gland-ovipositor (PG-OV) transcriptome of females of the fall armyworm, Spodoptera frugiperda, a pest of many crops. We annotated a total of 26 candidate chemosensory receptor genes, including 12 odorant receptors (ORs), 4 gustatory receptors (GRs), and 10 ionotropic receptors (IRs). The relatedness of these chemosensory receptors with those from other insect species was predicted by phylogenetic analyses, and specific genes, including pheromone receptors, ORco, CO(2) receptors, sugar receptors, and IR co-receptors, were reported. Although real-time quantitative-PCR analyses of annotated genes revealed that OR and IR genes were mainly expressed in S. frugiperda antennae, two ORs and two IRs expressed in antennae were also highly expressed in the PG-OV. Similarly, GR genes were mainly expressed in the proboscis, but two were also highly expressed in the PG-OV. Our study provides the first large-scale description of chemosensory receptors in the PG-OV of S. frugiperda and provides a foundation for exploring the chemoreception mechanisms of PG-OV in S. frugiperda and in other moth species" |
| Keywords: | Spodoptera frugiperda gustatory receptor ionotropic receptor odorant receptor pheromone gland-ovipositor real-time quantitative PCR transcriptome; |
| Notes: | "PubMed-not-MEDLINESun, Ya-Lan Dong, Jun-Feng Yang, Hai-Bo Li, Ding-Xu Tian, Cai-Hong eng 2021YFD1400701/National Key R& D Programs of China/ 201300111500/Major Special Science and Technology Project of Henan Province/ 13480064/Doctoral Scientific Research Foundation of Henan University of Science and Technology/ Switzerland 2022/05/28 Insects. 2022 May 21; 13(5):481. doi: 10.3390/insects13050481" |
