| Title: | Comparison of gas chromatography-combustion-mass spectrometry and gas chromatography-flame ionization detector for the determination of fatty acid methyl esters in biodiesel without specific standards |
| Author(s): | Sobrado LA; Freije-Carrelo L; Moldovan M; Encinar JR; Alonso JI; |
| Address: | "Department of Physical and Analytical Chemistry, University of Oviedo, Julian Claveria 8, 33006, Oviedo, Spain. Department of Physical and Analytical Chemistry, University of Oviedo, Julian Claveria 8, 33006, Oviedo, Spain. Electronic address: ruizencinar@uniovi.es. Department of Physical and Analytical Chemistry, University of Oviedo, Julian Claveria 8, 33006, Oviedo, Spain. Electronic address: jiga@uniovi.es" |
| DOI: | 10.1016/j.chroma.2016.06.033 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "GC-FID has been effectively used as a universal quantification technique for volatile organic compounds for a long time. In most cases, the use of the ECN allows for quantification by GC-FID without external calibration using only the response of a single internal standard. In this paper we compare the performance characteristics of GC-FID with those of post-column (13)C Isotope Dilution GC-Combustion-MS for the absolute quantification of organic compounds without the need for individual standards. For this comparison we have selected the quantification of FAMEs in biodiesel. The selection of the right internal standard was critical for GC-FID even when ECN were considered. On the other hand, the nature of the internal standard was not relevant when GC-Combustion-MS was employed. The proposed method was validated with the analysis of the certified reference material SRM 2772 and comparative data was obtained on real biodiesel samples. The analysis of the SRM 2772 biodiesel provided recoveries in the range 100.6-103.5% and 96.4-103.6% for GC-combustion-MS and GC-FID, respectively. The detection limit for GC-combustion-MS was found to be 4.2ng compound/g of injected sample. In conclusion, the quantitative performance of GC-Combustion-MS compared satisfactorily with that of GC-FID constituting a viable alternative for the quantification of organic compounds without the need for individual standards" |
| Keywords: | "Biofuels/*analysis Calibration Chromatography, Gas/methods Esters Fatty Acids/*analysis Flame Ionization Gas Chromatography-Mass Spectrometry/methods Limit of Detection Volatile Organic Compounds/*analysis Absolute quantification Biodiesel FAMEs Gc-fid GC;" |
| Notes: | "MedlineSobrado, Laura Alonso Freije-Carrelo, Laura Moldovan, Mariella Encinar, Jorge Ruiz Alonso, J Ignacio Garcia eng Comparative Study Netherlands 2016/07/03 J Chromatogr A. 2016 Jul 29; 1457:134-43. doi: 10.1016/j.chroma.2016.06.033. Epub 2016 Jun 14" |
