| Title: | Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells of Crassostrea gigas: A Novel Function of Shell Matrix Proteins |
| Author(s): | Sedanza MG; Yoshida A; Kim HJ; Yamaguchi K; Osatomi K; Satuito CG; |
| Address: | "Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki 852-8521, Japan. Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, Iloilo 5023, Philippines. Organization for Marine Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "The global decline of natural oyster populations emphasizes the need to improve our understanding of their biology. Understanding the role of chemical cues from conspecifics on how oysters occupy appropriate substrata is crucial to learning about their evolution, population dynamics, and chemical communication. Here, a novel role of a macromolecular assembly of shell matrix proteins which act as Crassostrea gigas Settlement Pheromone Protein Components in adult shells is demonstrated as the biological cue responsible for gregarious settlement on conspecifics. A bioassay-guided fractionation approach aided by biochemical and molecular analyses reveals that Gigasin-6 isoform X1 and/or X2 isolated from adult shells is the major inducing cue for larval settlement and may also play a role in postlarva-larva settlement interactions. Other isolated Stains-all-stainable acidic proteins may function as a co-factor and a scaffold/structural framework for other matrix proteins to anchor within this assembly and provide protection. Notably, conspecific cue-mediated larval settlement induction in C. gigas presents a complex system that requires an interplay of different glycans, disulfide bonds, amino acid groups, and phosphorylation crosstalk for recognition. These results may find application in the development of oyster aquacultures which could help recover declining marine species and as targets of anti-fouling agents" |
| Keywords: | Acids/metabolism Animal Shells/metabolism Animals *Crassostrea Cues Larva Pheromones/metabolism/pharmacology biomineralization conspecific cues lectin-glycan interaction marine chemical ecology oyster larval ecology post-translational modifications settle; |
| Notes: | "MedlineSedanza, Mary Grace Yoshida, Asami Kim, Hee-Jin Yamaguchi, Kenichi Osatomi, Kiyoshi Satuito, Cyril Glenn eng JSPS KAKENHI No. 19K06189/Japan Society for the Promotion of Science/ Switzerland 2022/09/10 Int J Mol Sci. 2022 Aug 29; 23(17):9816. doi: 10.3390/ijms23179816" |
