| Title: | Methanethiol Consumption and Hydrogen Sulfide Production by the Thermoacidophilic Methanotroph Methylacidiphilum fumariolicum SolV |
| Author(s): | Schmitz RA; Mohammadi SS; van Erven T; Berben T; Jetten MSM; Pol A; Op den Camp HJM; |
| Address: | "Department of Microbiology, Radboud Institute for Biological and Environmental Research, Radboud University, Nijmegen, Netherlands. Environmental Chemistry, Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, Zurich, Switzerland" |
| DOI: | 10.3389/fmicb.2022.857442 |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Methanotrophs aerobically oxidize methane to carbon dioxide to make a living and are known to degrade various other short chain carbon compounds as well. Volatile organic sulfur compounds such as methanethiol (CH(3)SH) are important intermediates in the sulfur cycle. Although volatile organic sulfur compounds co-occur with methane in various environments, little is known about how these compounds affect methanotrophy. The enzyme methanethiol oxidase catalyzing the oxidation of methanethiol has been known for decades, but only recently the mtoX gene encoding this enzyme was identified in a methylotrophic bacterium. The presence of a homologous gene in verrucomicrobial methanotrophs prompted us to examine how methanotrophs cope with methanethiol. Here, we show that the verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV consumes methanethiol and produces H(2)S, which is concurrently oxidized. Consumption of methanethiol is required since methanethiol inhibits methane oxidation. Cells incubated with approximately 15 muM methanethiol from the start clearly showed inhibition of growth. After depletion of methanethiol, growth resumed within 1 day. Genes encoding a putative methanethiol oxidase were found in a variety of methanotrophs. Therefore, we hypothesize that methanethiol degradation is a widespread detoxification mechanism in methanotrophs in a range of environments" |
| Keywords: | Methylacidiphilum hydrogen sulfide methanethiol methanotrophs sulfur cycle thermoacidophile; |
| Notes: | "PubMed-not-MEDLINESchmitz, Rob A Mohammadi, Sepehr S van Erven, Timo Berben, Tom Jetten, Mike S M Pol, Arjan Op den Camp, Huub J M eng Switzerland 2022/04/16 Front Microbiol. 2022 Mar 28; 13:857442. doi: 10.3389/fmicb.2022.857442. eCollection 2022" |
