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Mol Cell Biol

Title:		Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth
Author(s):		Roth AF; Nelson B; Boone C; Davis NG;
Address:		"Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan 48201, USA"
Journal Title:		Mol Cell Biol
Year:		2000
Volume:		20
Issue:		23
Page Number:		8815 - 8825
DOI:		10.1128/MCB.20.23.8815-8825.2000
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The inappropriate expression of the a-factor pheromone receptor (Ste3p) in the MATa cell leads to a striking inhibition of the yeast pheromone response, the result of a functional interaction between Ste3p and some MATa-specific protein. The present work identifies this protein as Asg7p. Normally, expression of Ste3p and Asg7p is limited to distinct haploid mating types, Ste3p to MATalpha cells and Asg7p to MATa cells. Artificial coexpression of the two in the same cell, either a or alpha, leads to dramatic inhibition of the pheromone response. Ste3p-Asg7p coexpression also perturbs the membrane trafficking of Ste3p: Ste3p turnover is slowed, a result of an Asg7p-mediated retardation of the secretory delivery of the newly synthesized receptor to the plasma membrane. However, in the absence of ectopic Ste3p expression, the asg7Delta mutation is without consequence either for pheromone signaling or overall mating efficiency of a cells. Indeed, the sole phenotype that can be assigned to MATa asg7Delta cells is observed following zygotic fusion to its alpha mating partner. Though formed at wild-type efficiency, zygotes from these pairings are morphologically abnormal. The pattern of growth is deranged: emergence of the first mitotic bud is delayed, and, in its place, growth is apparently diverted into a novel structure superficially resembling the polarized mating projection characteristic of haploid cells responding to pheromone. Together these results suggest a mechanism in which, following the zygotic fusion event, Ste3p and Asg7p gain access to one another and together act to repress the pheromone response, promoting the transition of the new diploid cell to vegetative growth"
Keywords:		"Cell Compartmentation Diploidy Gene Expression Regulation, Fungal Haploidy Mating Factor Peptides Pheromones/*metabolism Receptors, Cell Surface/*metabolism *Receptors, G-Protein-Coupled Receptors, Mating Factor *Receptors, Pheromone Repressor Proteins/*m;"
Notes:		"MedlineRoth, A F Nelson, B Boone, C Davis, N G eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2000/11/14 Mol Cell Biol. 2000 Dec; 20(23):8815-25. doi: 10.1128/MCB.20.23.8815-8825.2000"