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Genetics

Title:		Mating-type genes from the homothallic fungus Sordaria macrospora are functionally expressed in a heterothallic ascomycete
Author(s):		Poggeler S; Risch S; Kuck U; Osiewacz HD;
Address:		"Lehrstuhl fur Allgemeine Botanik, Ruhr-Universitat, Bochum, Germany"
Journal Title:		Genetics
Year:		1997
Volume:		147
Issue:		2
Page Number:		567 - 580
DOI:		10.1093/genetics/147.2.567
ISSN/ISBN:		0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"Homokaryons from the homothallic ascomycte Sordaria macrospora are able to enter the sexual pathway and to form fertile fruiting bodies. To analyze the molecular basis of homothallism and to elucidate the role of mating-products during fruiting body development, we cloned and sequenced the entire S. macrospora mating-type locus. Comparison of the Sordaria mating-type locus with mating-type idiomorphs from the heterothallic ascomycetes Neurospora crassa and Podospora anserina revealed that sequences from both idiomorphs (A/a and mat-/mat+, respectively) are contiguous in S. macrospora. DNA sequencing of the S. macrospora mating-type region allowed the identification of four open reading frames (ORFs), which were termed Smt-a1, SmtA-1, SmtA-2 and SmtA-3. While Smt-a1, SmtA-1, and SmtA-2 show strong sequence similarities with the corresponding N. crassa mating-type ORFs, SmtA-3 has a chimeric character. It comprises sequences that are similar to the A and a mating-type idiomorph from N. crassa. To determine functionality of the S. macrospora mating-type genes, we show that all ORFs are transcriptionally expressed. Furthermore, we transformed the S. macrospora mating-type genes into mat- and mat+ strains of the closely related heterothallic fungus P. anserina. The transformation experiments show that mating-type genes from S. macrospora induce fruiting body formation in P. anserina"
Keywords:		"Amino Acid Sequence Ascomycota/*genetics Base Sequence Cloning, Molecular DNA, Complementary Lipoproteins/*genetics Molecular Sequence Data Pheromones Polymerase Chain Reaction Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Sequence Homol;"
Notes:		"MedlinePoggeler, S Risch, S Kuck, U Osiewacz, H D eng Research Support, Non-U.S. Gov't 1997/10/23 Genetics. 1997 Oct; 147(2):567-80. doi: 10.1093/genetics/147.2.567"