| Title: | Development of a method based on on-line reversed phase liquid chromatography and gas chromatography coupled by means of an adsorption-desorption interface for the analysis of selected chiral volatile compounds in methyl jasmonate treated strawberries |
| Author(s): | Pena Moreno F; Blanch GP; Flores G; Ruiz Del Castillo ML; |
| Address: | "Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Cientificas (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain" |
| DOI: | 10.1016/j.chroma.2009.10.037 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "A method based on the use of the through oven transfer adsorption-desorption (TOTAD) interface in on-line coupling between reversed phase liquid chromatography and gas chromatography (RPLC-GC) for the determination of chiral volatile compounds was developed. In particular, the method was applied to the study of the influence of methyl jasmonate (MJ) treatment on the production and enantiomeric composition of selected aroma compounds in strawberry. The compounds studied were ethyl 2-methylbutanoate, linalool and 4-hydroxy-2,5-dimethyl-3(2H)-furanone (i.e. furaneol), which were examined on days 3, 6 and 9 after treatment. The method developed resulted in relative standard deviations (RSDs) of 21.6%, 8.1% and 9.8% and limits of detection (LD) of 0.04, 0.07 and 0.02mg/l for ethyl 2-methylbutanoate, linalool and furaneol, respectively. The application of the RPLC-TOTAD-GC method allowed higher levels of ethyl 2-methylbutanoate, linalool and furaneol to be detected, particularly after 9 days of treatment. Besides, MJ demonstrated to affect the enantiomeric distribution of ethyl 2-methylbutanoate. On the contrary, the enantiomeric composition of linalool and furaneol kept constant in both control and MJ-treated strawberries throughout the study. These results are discussed" |
| Keywords: | "Acetates/*pharmacology Acyclic Monoterpenes Adsorption Butyrates/analysis Chromatography, Gas/*methods Chromatography, Reverse-Phase/*methods Cyclopentanes/*pharmacology Fragaria/*chemistry/drug effects Fruit/*chemistry/drug effects Furans/analysis Monote;" |
| Notes: | "Medlinede la Pena Moreno, Fernando Blanch, Gracia Patricia Flores, Gema Ruiz Del Castillo, Maria Luisa eng Research Support, Non-U.S. Gov't Netherlands 2009/11/03 J Chromatogr A. 2010 Feb 12; 1217(7):1083-8. doi: 10.1016/j.chroma.2009.10.037. Epub 2009 Oct 20" |
