« Previous Abstract"[Morphology, anatomy, ontogeny and chemical composition of inflorescences volatile secondary metabolites of Lippia alba (Verbenaceae) at three stages of development]"    Next Abstract"Insect Rearing Techniques for Biological Control Programs, a Component of Sustainable Agriculture in Brazil" »

EMBO J

Title:		Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium
Author(s):		Parra-Lopez C; Baer MT; Groisman EA;
Address:		"Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110"
Journal Title:		EMBO J
Year:		1993
Volume:		12
Issue:		11
Page Number:		4053 - 4062
DOI:		10.1002/j.1460-2075.1993.tb06089.x
ISSN/ISBN:		0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking)
Abstract:		"The innate immunity of vertebrates and invertebrates to microbial infection is mediated in part by small cationic peptides with antimicrobial activity. Successful pathogens have evolved mechanisms to withstand the antibiotic activity of these molecules. We have isolated a set of genes from Salmonella typhimurium which are required for virulence and resistance to the antimicrobial peptides melittin and protamine. Sequence analysis of a 5.7 kb segment from the wild-type plasmid conferring resistance to protamine contained five open reading frames: sapA, sapB, sapC, sapD and sapF, organized in an operon structure and transcribed as a 5.3 kb mRNA. SapD and SapF exhibited similarity with the 'ATP binding cassette' family of transporters including the bacterial Opp and SpoOK, involved in the uptake of oligopeptides; the yeast STE6, necessary for the export of a peptide pheromone; and the mammalian mdr, which mediates resistance to chemotherapeutic agents in cancer cells. SapA showed identity with other periplasmic solute binding proteins involved in peptide transport. The SapABCDF system constitutes a novel transporter for enteric bacteria and the first one harboring a periplasmic component with a role in virulence"
Keywords:		"ATP-Binding Cassette Transporters/genetics Adenosine Triphosphate/metabolism Amino Acid Sequence Anti-Bacterial Agents/*pharmacology Base Sequence Biological Transport Cloning, Molecular Drug Resistance, Microbial/genetics Enterobacteriaceae/genetics *Gen;"
Notes:		"MedlineParra-Lopez, C Baer, M T Groisman, E A eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 1993/11/01 EMBO J. 1993 Nov; 12(11):4053-62. doi: 10.1002/j.1460-2075.1993.tb06089.x"