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Curr Genet

Title:		A dedicated glyceraldehyde-3-phosphate dehydrogenase is involved in the biosynthesis of volatile sesquiterpenes in Trichoderma virens-evidence for the role of a fungal GAPDH in secondary metabolism
Author(s):		Pachauri S; Chatterjee S; Kumar V; Mukherjee PK;
Address:		"Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India. Homi Bhabha National Institute, Anushaktinagar, Mumbai, 400094, India. Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India. Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India. prasunmukherjee1@gmail.com"
Journal Title:		Curr Genet
Year:		2019
Volume:		20180725
Issue:		1
Page Number:		243 - 252
DOI:		10.1007/s00294-018-0868-y
ISSN/ISBN:		1432-0983 (Electronic) 0172-8083 (Linking)
Abstract:		"Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyses the sixth step of glycolysis, and is also known to perform other (moonlighting) activities in animal cells. We have earlier identified an additional GAPDH gene in Trichoderma virens genome. This gene is consistently associated with the vir cluster responsible for biosynthesis of a range of volatile sesquiterpenes in Trichoderma virens. This gene is also associated with an orthologous gene cluster in Aspergillus spp. Both glycolytic GAPDH and the vir cluster-associated GAPDH show more than 80% similarity with essentially conserved NAD(+) cofactor- and substrate-binding sites. However, a conserved indel is consistently present only in GAPDH associated with the vir cluster, both in T. virens and Aspergillus spp. Using gene knockout, we demonstrate here that the vir cluster-associated GAPDH is involved in biosynthesis of volatile sesquiterpenes in T. virens. We thus, for the first time, elucidate the non-glycolytic role of a GAPDH in a fungal system, and also prove for the first time that a GAPDH, a primary metabolism protein, is involved in secondary metabolism"
Keywords:		"Fungal Proteins/classification/*genetics/metabolism Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Fungal Glyceraldehyde-3-Phosphate Dehydrogenases/classification/*genetics/metabolism Isoenzymes/genetics/metabolism Multigene Family/genet;"
Notes:		"MedlinePachauri, Shikha Chatterjee, Suchandra Kumar, Vinay Mukherjee, Prasun K eng 2018/07/27 Curr Genet. 2019 Feb; 65(1):243-252. doi: 10.1007/s00294-018-0868-y. Epub 2018 Jul 25"