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Arch Microbiol

Title:		Cultured and uncultured microbial community associated with biogas production in anaerobic digestion processes
Author(s):		Ottoni JR; Bernal SPF; Marteres TJ; Luiz FN; Dos Santos VP; Mari AG; Somer JG; de Oliveira VM; Passarini MRZ;
Address:		"Laboratorio de Biotecnologia Ambiental, Universidade Federal da Integracao Latino-Americana - Unila., Jd Universitario, Av. Tarquinio Joslin dos Santos, 1000, Foz do Iguacu, PR, 85870-650, Brazil. Centro Internacional de Energias Renovaveis - Biogas (CIBiogas-ER), Av. Tancredo Neves, 6731, Itaipu, Foz do Iguacu, PR, Brazil. CPQBA/UNICAMP - Divisao de Recursos Microbianos, Campinas, Av. Alexandre Cazelatto, 999. Betel, Paulinia, SP, Brazil. Laboratorio de Biotecnologia Ambiental, Universidade Federal da Integracao Latino-Americana - Unila., Jd Universitario, Av. Tarquinio Joslin dos Santos, 1000, Foz do Iguacu, PR, 85870-650, Brazil. michel.passarini@unila.edu.com.br"
Journal Title:		Arch Microbiol
Year:		2022
Volume:		20220519
Issue:		6
Page Number:		340 -
DOI:		10.1007/s00203-022-02819-8
ISSN/ISBN:		1432-072X (Electronic) 0302-8933 (Linking)
Abstract:		"The search for sustainable development has increased interest in the improvement of technologies that use renewable energy sources. One of the alternatives in the production of renewable energy comes from the use of waste including urban solids, animal excrement from livestock, and biomass residues from agro-industrial plants. These materials may be used in the production of biogas, making its production highly sustainable and environmentally friendly. The present study aimed to evaluate the cultivated and uncultivated microbial community from a substrate (starter) used as an adapter for biogas production in anaerobic digestion processes. 16S rDNA metabarcoding revealed the domain of bacteria belonging to the phyla Firmicutes, Bacteroidota, Chloroflexi and Synergistota. The methanogenic group was represented by the phyla Halobacterota and Euryarchaeota. Through 16S rRNA sequencing of isolates recovered from the starter culture, the genera Rhodococcus (Actinobacteria phylum), Vagococcus, Lysinibacillus, Niallia, Priestia, Robertmurraya, Proteiniclasticum (Firmicutes phylum), and Luteimonas (Proteobacteria phylum) were identified, genera that were not observed in the metabarcoding data. The volatile solids, volatile organic acids, and total inorganic carbon reached 659.10 g kg(-1), 717.70 g kg(-1), 70,005.0 g kg(-1), respectively. The cultured groups are involved in the metabolism of sugars and other compounds derived from lignocellulosic material, as well as in anaerobic methane production processes. The results demonstrate that culture-dependent approaches, such as isolation and sequencing, and culture-independent studies, such as the Metabarcoding approach, are complementary methodologies that, when integrated provide robust and comprehensive information about the microbial communities involved in processes of the production of biogas in anaerobic digestion processes"
Keywords:		"Anaerobiosis Animals Bacteria *Biofuels Bioreactors/microbiology Methane/metabolism *Microbiota RNA, Ribosomal, 16S/genetics/metabolism 16S rRNA Anaerobic digestion processes Culture-dependent Culture-independent approaches Metabarcoding;"
Notes:		"MedlineOttoni, Julia Ronzella Bernal, Suzan Prado Fernandes Marteres, Tiago Joelzer Luiz, Franciele Natividade Dos Santos, Viviane Piccin Mari, Angelo Gabriel Somer, Juliana Gaio de Oliveira, Valeria Maia Passarini, Michel Rodrigo Zambrano eng PRPPG 105/2020/Latin America and the Caribbean Priority Institutional Program/ EDITAL PRPPG n degrees 80/2019/Researcher Integration Assistance Program (PAIP)/ Germany 2022/05/20 Arch Microbiol. 2022 May 19; 204(6):340. doi: 10.1007/s00203-022-02819-8"