| Title: | A constitutively active G-protein-coupled receptor causes mating self-compatibility in the mushroom Coprinus |
| Author(s): | Olesnicky NS; Brown AJ; Dowell SJ; Casselton LA; |
| Address: | "Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, UK" |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "In the mushroom Coprinus cinereus, the multiallelic B mating type genes are predicted to encode a large family of seven-transmembrane domain receptors and CaaX-modified pheromones. We have shown that a single amino acid change Q229P in transmembrane domain VI of one receptor confers a self-compatible mating phenotype. Using a heterologous yeast assay, we have demonstrated that this C.cinereus pheromone receptor is a G-protein-coupled receptor and that the Q229P mutation is constitutively activating. A C.cinereus pheromone precursor was processed to an active species specifically in yeast MATa cells and activated the co-expressed wild-type receptor. Yeast cells expressing the wild-type receptor were used to test the activity of synthetic peptides, enabling us to predict the structure of the mature C.cinereus pheromone and to show that the Q229P mutation does not compromise normal receptor function" |
| Keywords: | "Chemoreceptor Cells/*metabolism Coprinus/metabolism/*physiology GTP-Binding Proteins/*metabolism Gene Expression Genes, Fungal Genes, Mating Type, Fungal Molecular Sequence Data Mutation Peptides/chemistry/pharmacology Phenotype Pheromones/metabolism Repr;" |
| Notes: | "MedlineOlesnicky, N S Brown, A J Dowell, S J Casselton, L A eng Research Support, Non-U.S. Gov't England 1999/05/18 EMBO J. 1999 May 17; 18(10):2756-63. doi: 10.1093/emboj/18.10.2756" |
