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Anal Chem

Title:		Analysis of Drosophila lipids by matrix-assisted laser desorption/ionization mass spectrometric imaging
Author(s):		Niehoff AC; Kettling H; Pirkl A; Chiang YN; Dreisewerd K; Yew JY;
Address:		"Temasek Life Sciences Laboratory , 1 Research Link, Singapore 117604"
Journal Title:		Anal Chem
Year:		2014
Volume:		20141105
Issue:		22
Page Number:		11086 - 11092
DOI:		10.1021/ac503171f
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"Drosophila melanogaster is a major model organism for numerous lipid-related diseases. While comprehensive lipidomic profiles have been generated for D. melanogaster, little information is available on the localization of individual lipid classes and species. Here, we show the use of matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) to profile lipids in D. melanogaster tissue sections. The preparation of intact cryosections from whole insects presents a challenge due to the brittle hydrophobic cuticle surrounding the body and heterogeneous tissue types beneath the cuticle. However, the introduction of a novel sucrose infiltration step and gelatin as an embedding media greatly improved the quality of tissue sections. We generated MS image profiles of six major lipid classes: phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and triacylglycerides. In addition, signals corresponding to two male-specific sex pheromones were detected in the ejaculatory bulb, a specialized site of pheromone production. MSI performed with 35 mum lateral resolution provided high sensitivity detection of at least 92 different lipid species, based on exact mass. In contrast, MSI with 10 mum lateral resolution enabled the detection of 36 lipid species but allowed lipid profiling of individual organs. The ability to localize lipid classes in intact sections from whole Drosophila provides a powerful tool for characterizing the effects of diet, age, stress, and environment on lipid production and distribution"
Keywords:		"Animals Drosophila melanogaster/*chemistry Lipids/*analysis/*chemistry *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;"
Notes:		"MedlineNiehoff, Ann-Christin Kettling, Hans Pirkl, Alexander Chiang, Yin Ning Dreisewerd, Klaus Yew, Joanne Y eng Research Support, Non-U.S. Gov't 2014/10/21 Anal Chem. 2014 Nov 18; 86(22):11086-92. doi: 10.1021/ac503171f. Epub 2014 Nov 5"