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J Plant Physiol

Title:		CsV03-3 is a member of a novel gene family from citrus that encodes a protein with DNA binding activity and whose expression is responsive to defense signals and abiotic stress
Author(s):		Mozoruk J; Hunnicutt LE; Hunter WB; Bausher MG;
Address:		"Horticulture & Breeding Research Unit, USDA ARS US Horticultural Research Laboratory, 2001 S. Rock Rd. Ft. Pierce, FL 34945, USA"
Journal Title:		J Plant Physiol
Year:		2008
Volume:		20070507
Issue:		5
Page Number:		531 - 543
DOI:		10.1016/j.jplph.2007.01.009
ISSN/ISBN:		0176-1617 (Print) 0176-1617 (Linking)
Abstract:		"We have identified a novel gene designated CsV03-3 from Citrus sinensis (L.) Osbeck that encodes a 50 amino acid polypeptide with a predicted molecular mass of 5.4kDa and a pI of 3.7. CsV03-3 expression is up-regulated by application of methyl jasmonate, salicylic acid, and abscisic acid as well as by abiotic stress and insect herbivory. CsV03-3 belongs to a small gene family consisting of at least three other closely related members (CsV03-1, CsV03-2, and CsV03-4) whose expression is also responsive to phytohormone application and abiotic stress. Sequence similarity searches of the public databases were unsuccessful in finding sequence homologs to CsV03-3 or any CsV03 family member; however, structural prediction models coupled with model comparison to Protein Data Bank folds indicated that the predicted polypeptide encoded by CsV03-3 has structural similarity to proteins with nucleic acid binding activity. Gel mobility shift assays performed on recombinant CsV03-3 protein demonstrated active binding with dsDNA and, to a lesser extent, ssDNA. Based on the phytohormone-inducible expression patterns, the ability to bind nucleic acids, and the lack of significant sequence homology to public databases, we propose that CsV03-3 and its related homologs defines a new class of nucleic acid binding proteins that are responsive to defense and stress signaling in woody perennials"
Keywords:		"Base Sequence Blotting, Southern Citrus/genetics/*metabolism Cyclopentanes/pharmacology DNA-Binding Proteins/chemistry/genetics/*metabolism Electrophoretic Mobility Shift Assay Gene Expression Regulation, Plant/drug effects Molecular Sequence Data Oxylipi;"
Notes:		"MedlineMozoruk, Jerry Hunnicutt, Laura E Hunter, Wayne B Bausher, Michael G eng Germany 2007/05/08 J Plant Physiol. 2008; 165(5):531-43. doi: 10.1016/j.jplph.2007.01.009. Epub 2007 May 7"