« Previous AbstractA fatty acid synthase gene (FASN3) from the integument tissue of Rhodnius prolixus contributes to cuticle water loss regulation    Next AbstractBiopolymer-encapsulated protein microcapsules spontaneously formed at the ionic liquid-water interface »

Zoolog Sci

Title:		Cloning and Characterization of Cyp7a1 and Cyp27a1 Genes from the Non-Parasitic Japanese Lamprey Lethenteron reissneri
Author(s):		Morii M; Hebiguchi T; Watanabe R; Yoshino H; Mezaki Y;
Address:		"Department of Pediatric Surgery, Akita University Graduate School of Medicine, Akita-city, Akita 010-8543, Japan. Department of Laboratory Medicine, the Jikei University Graduate School of Medicine, Minato-ku, Tokyo 105-8461, Japan, mezaki@jikei.ac.jp"
Journal Title:		Zoolog Sci
Year:		2023
Volume:		40
Issue:		3
Page Number:		208 - 218
DOI:		10.2108/zs220072
ISSN/ISBN:		0289-0003 (Print) 0289-0003 (Linking)
Abstract:		"Two cytochrome P450 genes homologous to human CYP7A1 and CYP27A1 were cloned from the non-parasitic Japanese lamprey Lethenteron reissneri. Lamprey cyp7a1 mRNA had varied expression levels among individuals: about four orders of magnitude differences in larval liver and nearly three orders of magnitude differences in male adult liver. Overexpressed Cyp7a1 protein tagged with green fluorescent protein (GFP) was localized to the endoplasmic reticulum. Lamprey cyp27a1 mRNA had relatively constant expression levels: within two orders of magnitude differences in larvae and adult liver and intestine. GFP-tagged Cyp27a1 protein was localized to mitochondria. The expression profiles of lamprey cyp7a1 and cyp27a1 genes and the cellular localizations of their products were in good agreement with their counterparts in mammals, where these two P450s catalyze initial hydroxylation reactions of cholesterol in classical and alternative pathways of bile acid synthesis, respectively. The cyp7a1 mRNA levels in adult male liver showed significant negative correlations to both body weight and total length of the animal, implying the involvement of the gene in the production of female-attractive pheromones in sexually matured male livers. The lamprey Cyp7a1 contains a long extension of 116 amino acids between helices D and E of the protein. Possible roles of this extension in regulating the enzymatic activity of lamprey Cyp7a1 are discussed"
Keywords:		"Animals Female Male Bile Acids and Salts/metabolism Cholestanetriol 26-Monooxygenase/genetics/metabolism Cholesterol 7-alpha-Hydroxylase/genetics/metabolism Cloning, Molecular Cytochrome P-450 Enzyme System/genetics/metabolism *Lampreys/genetics/metabolis;"
Notes:		"MedlineMorii, Mayako Hebiguchi, Taku Watanabe, Ryo Yoshino, Hiroaki Mezaki, Yoshihiro eng Japan 2023/05/31 Zoolog Sci. 2023 Jun; 40(3):208-218. doi: 10.2108/zs220072"