| Title: | Ex vivo emission of volatile organic compounds from gastric cancer and non-cancerous tissue |
| Author(s): | Mochalski P; Leja M; Gasenko E; Skapars R; Santare D; Sivins A; Aronsson DE; Ager C; Jaeschke C; Shani G; Mitrovics J; Mayhew CA; Haick H; |
| Address: | "Institute for Breath Research, University of Innsbruck, Rathausplatz 4, A-6850 Dornbirn, Austria. Institute of Chemistry, Jan Kochanowski University, Swietokrzyska 15G, PL-25406 Kielce, Poland" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "The presence of certain volatile organic compounds (VOCs) in the breath of patients with gastric cancer has been reported by a number of research groups; however, the source of these compounds remains controversial. Comparison of VOCs emitted from gastric cancer tissue to those emitted from non-cancerous tissue would help in understanding which of the VOCs are associated with gastric cancer and provide a deeper knowledge on their generation. Gas chromatography with mass spectrometric detection (GC-MS) coupled with head-space needle trap extraction (HS-NTE) as the pre-concentration technique, was used to identify and quantify VOCs released by gastric cancer and non-cancerous tissue samples collected from 41 patients during surgery. Excluding contaminants, a total of 32 VOCs were liberated by the tissue samples. The emission of four of them (carbon disulfide, pyridine, 3-methyl-2-butanone and 2-pentanone) was significantly higher from cancer tissue, whereas three compounds (isoprene, gamma-butyrolactone and dimethyl sulfide) were in greater concentration from the non-cancerous tissues (Wilcoxon signed-rank test, p < 0.05). Furthermore, the levels of three VOCs (2-methyl-1-propene, 2-propenenitrile and pyrrole) were correlated with the occurrence of H. pylori; and four compounds (acetonitrile, pyridine, toluene and 3-methylpyridine) were associated with tobacco smoking. Ex vivo analysis of VOCs emitted by human tissue samples provides a unique opportunity to identify chemical patterns associated with a cancerous state and can be considered as a complementary source of information on volatile biomarkers found in breath, blood or urine" |
| Keywords: | Adult Aged Biomarkers/analysis Breath Tests Female Gas Chromatography-Mass Spectrometry Humans Limit of Detection Male Middle Aged Reproducibility of Results Stomach Neoplasms/*metabolism Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineMochalski, Pawel Leja, Marcis Gasenko, Evita Skapars, Roberts Santare, Daiga Sivins, Armands Aronsson, Dan Erik Ager, Clemens Jaeschke, Carsten Shani, Gidi Mitrovics, Jan Mayhew, Christopher A Haick, Hossam eng Research Support, Non-U.S. Gov't England 2018/06/13 J Breath Res. 2018 Jul 30; 12(4):046005. doi: 10.1088/1752-7163/aacbfb" |
